Dose-dependent cytotoxicity of bismuth nanoparticles produced by LASiS in a reference mammalian cell line BALB/c 3T3
| Autor: | Thamile Luciane Reus, Ariane Caroline Campos Paschoal, Crisciele Kuligovski, Bruno Dallagiovanna, Alessandra Melo de Aguiar, Bruna H. Marcon, Arandi Ginane Bezerra, Thiago Neves Machado |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Programmed cell death BALB 3T3 Cells Cell Survival Metal Nanoparticles Apoptosis 02 engineering and technology Toxicology Mice 03 medical and health sciences medicine Animals Cytotoxic T cell Bovine serum albumin Cytotoxicity Cell damage Dose-Response Relationship Drug biology Chemistry General Medicine 021001 nanoscience & nanotechnology medicine.disease Molecular biology 030104 developmental biology Nanotoxicology biology.protein 0210 nano-technology Bismuth Fetal bovine serum |
| Zdroj: | Toxicology in Vitro. 53:99-106 |
| ISSN: | 0887-2333 |
| Popis: | Nanoparticles (NPs) have emerged as new potential tools for many applications in previous years. Among all types of NPs, bismuth NPs (BiNPs) have a very low cost and potential for many applications, ranging from medicine to industry. Although the toxic effects of bismuth have been studied, little is known about its toxicity at the nanoscale level. Therefore, in this study, we aimed to investigate the cytotoxic effects of BiNPs produced by laser ablation synthesis in solution (LASiS) in a reference mammalian cell line to evaluate their cytotoxicity (BALB/c 3 T3 cells). We also stabilized BiNPs in two different solutions: culture medium supplemented with fetal bovine serum (FBS) and bovine serum albumin (BSA). The cytotoxicity of BiNPs in culture medium (IC50:28.51 ± 9.96 μg/ml) and in BSA (IC50:25.54 ± 8.37 μg/ml) was assessed, and they were not significantly different. Second, the LD50 was predicted, and BiNPs were estimated as GHS class 4. We also found that cell death occurs due to apoptosis. By evaluating the interaction between BiNPs and cells at ultrastructural level, we suggest that cell death occurs once BiNPs are internalized. Additionally, we suggest that BiNPs cause cell damage because myelin figures were found inside cells that had internalized BiNPs. To date, this is the first study to assess the cytotoxicity of BiNPs produced by LASiS and to predict the possible LD50 and GHS class of BiNPs. |
| Databáze: | OpenAIRE |
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