Towards standardization of immune functional assays
Autor: | Karen Brengel-Pesce, Guy Oriol, Sophie Trouillet-Assant, William Mouton, Julien Textoris, Chloé Albert-Vega, François Bartolo, Alexandre Pachot, Mathilde Boccard, François Mallet, Jonathan Lopez |
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Přispěvatelé: | Virpath-Grippe, de l'émergence au contrôle -- Virpath-Influenza, from emergence to control (Virpath), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Joint Research Unit Hospices Civils de Lyon-bioMérieux, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Hospices Civils de Lyon (HCL), BIOMERIEUX, Physiopathologie de l'immunodépression associée aux réponses inflammatoires systémiques - EA 7426 (PI3), Université de Lyon-Université de Lyon, CCSD, Accord Elsevier, Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Physiopathologie de l'immunodépression associée aux réponses inflammatoires systémiques / Pathophysiology of Injury-induced Immunosuppression (PI3) |
Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Adult Male Standardization animal diseases medicine.medical_treatment [SDV]Life Sciences [q-bio] Immunology Host response Immune functional assay chemical and pharmacologic phenomena Clinical settings Adaptive Immunity Immune Dysfunction Cohort Studies 03 medical and health sciences Interferon-gamma 0302 clinical medicine Immune system Healthy volunteers Immunology and Allergy Medicine Humans Cells Cultured 030304 developmental biology Aged Immunoassay 0303 health sciences Immune status business.industry Reproducibility of Results Immunotherapy Middle Aged Reference Standards Healthy Volunteers Independent validation 3. Good health [SDV] Life Sciences [q-bio] 030104 developmental biology 030220 oncology & carcinogenesis Stimulation Female business Transcriptome 030215 immunology |
Zdroj: | Clinical Immunology Clinical Immunology, 2020, 210, pp.108312. ⟨10.1016/j.clim.2019.108312⟩ Clinical Immunology, Elsevier, 2020, 210, pp.108312-. ⟨10.1016/j.clim.2019.108312⟩ |
ISSN: | 1521-6616 1521-7035 |
Popis: | Recent advances in the immunotherapy field require evaluation of the immune function to adapt therapeutic decisions. Immune functional assays (IFA) are able to reveal the immune status and would be useful to further adapt/improve patient’s care. However, standardized methods are needed to implement IFA in clinical settings. We carried out an independent validation of a published method used to characterize the underlying host response to infectious conditions using an IFA. We evaluate the reproducibility and robustness of this IFA and associated readout using an independent healthy volunteers (HV) cohort. Expression of a 44 genes-signatures and IFNγ protein secretion and gene-expression was assessed after stimulation. We observed a strong host-response correlation between the two cohorts. We also highlight that standardized methods for immune function evaluation exist and could be implemented in larger-scale studies. This IFA could be a relevant tool to reveal innate/adaptive immune dysfunction in immune-related disorders patients. |
Databáze: | OpenAIRE |
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