Cross-sensitivity between interferon and uv in human cell strains: IFr, HEC-1, and CRL1200
| Autor: | Takayuki Kojima, Tsuguo Kuwata, Takashi Miki, Junichi Nishimaki, Nobuo Suzuki, Yoshiaki Takakubo |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
DNA Replication
Xeroderma pigmentosum DNA Repair Cell Survival Ultraviolet Rays Cross sensitivity Biology Cell Line Interferon Virology medicine Humans Gene Xeroderma Pigmentosum Cell growth Biological activity Human cell medicine.disease Molecular biology Kinetics Cell culture Interferon Type I Uterine Neoplasms Immunology Female Cell Division medicine.drug |
| Zdroj: | Virology. 135:20-29 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/0042-6822(84)90113-2 |
| Popis: | Correlation between susceptibility to the anticellular effect of human interferon (HuIFN) and ultraviolet (uv) lethality was examined in a set of isogeneous human cell lines (RSa and IFr cells), a human endometrial cancer cell line (HEC-1 cells), and a Xeroderma pigmentosum-derived fibroblast cell line ( CRL1200 cells). IFr cells, previously established as a HuIFN-alpha-resistant subline by exposing HuIFN-alpha- and uv-sensitive RSa cells to HuIFN-alpha preparations, appeared more refractory to uv than did the parent RSa cells in the cell proliferation and colony-formation studies. The extent of recovery from uv-inhibited total cellular DNA synthesis and uv-induced DNA-repair synthesis was enhanced to a greater extent in IFr cells than in RSa cells. The preexposure of RSa cells to HuIFN-alpha enhanced uv-induced DNA-repair replication activities. HEC-1 cells, which are reportedly totally refractory to HuIFN actions, appeared most resistant to uv, in all the tests. The uv-sensitive CRL1200 cells appeared highly susceptible to HuIFN-beta, in both cell proliferation and DNA-synthesis inhibition tests. These results support and extend our previous finding (N. Suzuki, J. Nishimaki , and T. Kuwata (1982). Mutat . Res. 106, 357-376) that susceptibility to the anticellular effect of HuIFN closely relates with uv lethality. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|