Scarcity ofars sequences isolated in a morphogenesis mutant of the yeastYarrowia lipolytica
| Autor: | Claude Gaillardin, Ph. Fournier, L. Guyaneux, M. Chasles |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
DNA Replication
Autonomously replicating sequence Restriction Mapping Mutant Bioengineering Biology Applied Microbiology and Biotechnology Biochemistry Transformation Genetic Plasmid Restriction map Yeasts Extrachromosomal DNA Genetics Cloning Molecular DNA Fungal Gene Library Genomic organization Base Sequence Yarrowia biology.organism_classification Yeast Mutation Saccharomycetales DNA Probes Plasmids Biotechnology |
| Zdroj: | Yeast. 7:25-36 |
| ISSN: | 1097-0061 0749-503X |
| DOI: | 10.1002/yea.320070104 |
| Popis: | Previous attempts is isolate autonomously replicating sequences (ars) from the dimorphic yeast Yarrowia lipolytica have been unsuccessful. We isolated a Fil- mutant unable to produce hyphae and growing only in a yeast form to facilitate ars isolation. This mutant was transformed with a Y. lipolytica DNA bank and several unstable clones were obtained. Extrachromosomal plasmids were evidenced in yeast, recovered in Escherichia coli and characterized by restriction mapping. They were able to retransform Fil- and Fil+ yeast strains at high frequency and transformants displayed a slightly unstable phenotype. The detailed analysis of the plasmids showed that only two different ars sequences had been isolated, each of them corresponding to a unique sequence in the Y. lipolytica genome. We concluded that functional ars sequences that can be cloned on plasmids are rare in this yeast. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text