Evaluation of [3H]thymidine uptake method for studying growth of spiroplasmas under various conditions
| Autor: | F O Bastian, B S Baliga, H M Pollock |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Microbiology (medical)
biology Microorganism Spiroplasma Colony Count Microbial Spiroplasma mirum medicine.disease_cause biology.organism_classification Tritium Culture Media chemistry.chemical_compound Chemically defined medium Biochemistry chemistry medicine Mollicutes Anaerobiosis Thymidine Anaerobic exercise Bacteria Research Article |
| Zdroj: | Journal of clinical microbiology. 26(10) |
| ISSN: | 0095-1137 |
| Popis: | [3H]thymidine uptake and colony counts are quantitative and inexpensive methods for studying Spiroplasma growth. Using these techniques, we demonstrated subtle effects on the growth of suckling mouse cataract agent of medium alterations, inoculum size, and freezing of cultures. In addition, suckling mouse cataract agent multiplied more actively under aerobic than under anaerobic conditions. These techniques have wide application for the study of Spiroplasma growth and will be useful for the development of a defined medium. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|