Improving skin integration around long-term percutaneous devices using fibrous scaffolds in a reconstructed human skin equivalent model
| Autor: | Nicole Bartnikowski, Shaun D. Gregory, Eleonore C.L. Bolle, Tony J. Parker, John F. Fraser, Parvathi Haridas, Tim R. Dargaville |
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| Rok vydání: | 2019 |
| Předmět: |
Scaffold
melt electrowriting Materials science Percutaneous Surface Properties Polyesters 0206 medical engineering Biomedical Engineering Nanofibers Human skin Biocompatible Materials 02 engineering and technology Biomaterials Dermal fibroblast Prosthesis Implantation 03 medical and health sciences Equivalent model Dermis Tissue engineering polycaprolactone medicine Humans Cells Cultured 030304 developmental biology Skin 0303 health sciences integumentary system Tissue Engineering Tissue Scaffolds percutaneous device Fibroblasts 020601 biomedical engineering 090300 BIOMEDICAL ENGINEERING dermis medicine.anatomical_structure Mechanical Tests Ex vivo Biomedical engineering |
| Zdroj: | Journal of Biomedical Materials Research Part B: Applied Biomaterials |
| ISSN: | 1552-4981 |
| Popis: | The interface between synthetic percutaneous devices and skin is a common area for bacterial infection, which may ultimately result in failure of the device. Better integration of percutaneous devices with skin may help reduce infection rates due to the creation of a dermal seal. However, the mismatch in material and chemical properties of devices and skin presents a challenge for closing the dermal gap at the skin–device interface. Here, we have used a tissue engineering approach to tissue integration by creating a highly fibrous poly(e-caprolactone) scaffold using melt electrowriting and seeding this with dermal fibroblasts, followed by maturation and insertion into a full-thickness defect made in an ex vivo skin model. The integration of seeded scaffolds was compared with controls including a non-seeded scaffold and a polymer tube with a smooth surface. Dermal fibroblast inclusion in the scaffold and epidermal upgrowth versus downgrowth/marsupialization around the device were used as measures of integration. Based on these measures, almost all pre-seeded scaffolds performed better than both the non-seeded scaffolds and smooth tubes. The hypothesis is that the fibroblasts act as a barrier to epithelial downward migration, and provide healthy tissue for nascent epidermal development. |
| Databáze: | OpenAIRE |
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