A member of the PLEIOTROPIC DRUG RESISTANCE family of ATP binding cassette transporters is required for the formation of a functional cuticle in Arabidopsis
| Autor: | Michael Bessire, Reinhard Jetter, Luis Carraça, Nadia Efremova, Jean-Pierre Métraux, Anne-Claude Jacquat, Alexander Yephremov, Guillaume Fabre, Christiane Nawrath, Sandra Borel, Yan Cao |
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| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
Genotype Cuticle Mutant Arabidopsis ATP Binding Cassette Transporter Subfamily G ATP-binding cassette transporter Flowers Plant Science Cutin 01 natural sciences Permeability Plant Epidermis Cell wall Membrane Lipids 03 medical and health sciences Cell Wall Gene Expression Regulation Plant Arabidopsis thaliana Research Articles Sequence Deletion 030304 developmental biology 0303 health sciences integumentary system biology Arabidopsis Proteins Cell Membrane fungi food and beverages Cell Biology Plants Genetically Modified biology.organism_classification Cell biology Plant Leaves Phenotype Plant cuticle Biochemistry ATP-Binding Cassette Transporters ATP Binding Cassette Transporter 1 010606 plant biology & botany |
| Zdroj: | The Plant cell |
| DOI: | 10.1105/tpc.111.083121 |
| Popis: | Although the multilayered structure of the plant cuticle was discovered many years ago, the molecular basis of its formation and the functional relevance of the layers are not understood. Here, we present the permeable cuticle1 (pec1) mutant of Arabidopsis thaliana, which displays features associated with a highly permeable cuticle in several organs. In pec1 flowers, typical cutin monomers, such as ω- hydroxylated fatty acids and 10,16-dihydroxypalmitate, are reduced to 40% of wild-type levels and are accompanied by the appearance of lipidic inclusions within the epidermal cell. The cuticular layer of the cell wall, rather than the cuticle proper, is structurally altered in pec1 petals. Therefore, a significant role for the formation of the diffusion barrier in petals can be attributed to this layer. Thus, pec1 defines a new class of mutants. The phenotypes of the pec1 mutant are caused by the knockout of ATP BINDING CASSETTEG32 (ABCG32), an ABC transporter from the PLEIOTROPIC DRUG RESISTANCE family that is localized at the plasma membrane of epidermal cells in a polar manner toward the surface of the organs. Our results suggest that ABCG32 is involved in the formation of the cuticular layer of the cell wall, most likely by exporting particular cutin precursors from the epidermal cell. |
| Databáze: | OpenAIRE |
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