A Universal Multi-Platform 3D Printed Bioreactor Chamber for Tendon Tissue Engineering
| Autor: | Adam J. Janvier, James R. Henstock, EG Canty-Laird |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
3d printed
tendon Computer science 0206 medical engineering Bioreactor Biomedical Engineering Medicine (miscellaneous) 3D printing Mechanical engineering 02 engineering and technology Tendon tissue lcsh:Biochemistry MSC Biomaterials 03 medical and health sciences lcsh:QD415-436 Multi platform 030304 developmental biology 0303 health sciences business.industry technology industry and agriculture Linear actuator equipment and supplies 020601 biomedical engineering Original Article Design and Manufacture of Tissue Engineered Products using Additive Manufacturing Techniques hydrogel business |
| Zdroj: | Journal of Tissue Engineering Journal of Tissue Engineering, Vol 11 (2020) |
| Popis: | A range of bioreactors use linear actuators to apply tensile forces in vitro, but differences in their culture environments can limit a direct comparison between studies. The widespread availability of 3D printing now provides an opportunity to develop a ‘universal’ bioreactor chamber that, with minimal exterior editing can be coupled to a wide range of commonly used linear actuator platforms, for example, the EBERS-TC3 and CellScale MCT6, resulting in a greater comparability between results and consistent testing of potential therapeutics. We designed a bioreactor chamber with six independent wells that was 3D printed in polylactic acid using an Ultimaker 2+ and waterproofed using a commercially available coating (XTC-3D), an oxirane resin. The cell culture wells were further coated with Sylgard-184 polydimethylsiloxane (PDMS) to produce a low-adhesion well surface. With appropriate coating and washing steps, all materials were shown to be non-cytotoxic by lactate dehydrogenase assay, and the bioreactor was waterproof, sterilisable and reusable. Tissue-engineered tendons were generated from human mesenchymal stem cells in a fibrin hydrogel and responded to 5% cyclic strain (0.5 Hz, 5 h/day, 21 days) in the bioreactor by increased production of collagen-Iα1 and decreased production of collagen-IIIα1. Calcification of the extracellular matrix was observed in unstretched tendon controls indicating abnormal differentiation, while tendons cultured under cyclic strain did not calcify and exhibited a tenogenic phenotype. The ease of manufacturing this bioreactor chamber enables researchers to quickly and cheaply reproduce this culture environment for use with many existing bioreactor actuator platforms by downloading the editable CAD files from a public database and following the manufacturing steps we describe. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|