Black Cohosh (Cimicifuga racemosa L.) Protects against Menadione-Induced DNA Damage through Scavenging of Reactive Oxygen Species: Bioassay-Directed Isolation and Characterization of Active Principles
| Autor: | Burdette, J. E., Chen, S. -N, Lu, Z. -Z, Xu, H., White, B. E. P., Fabricant, D. S., Liu, J., Fong, H. H. S., Farnsworth, N. R., Constantinou, Andreas I., Van Breemen, R. B., Pezzuto, J. M., Bolton, J. L. |
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| Přispěvatelé: | Constantinou, Andreas I. [0000-0003-0365-1821] |
| Rok vydání: | 2002 |
| Předmět: |
enzyme assay
Cimicifuga Antioxidant DPPH medicine.medical_treatment menopause antioxidant activity Menadione Gene Expression reactive oxygen metabolite cancer cell culture Antioxidants gel electrophoresis Ferulic acid chemistry.chemical_compound Tumor Cells Cultured Caffeic acid Methyl caffeate oxidative stress genetics DNA strand breakage breast tumor drug effect thin layer chromatography article Vitamin K 3 Free Radical Scavengers unclassified drug bioassay cimicifuga Receptors Estrogen Biochemistry plant extract caffeic acid derivative Cimicifuga racemosa (black cohosh Actaea racemosa) gallic acid General Agricultural and Biological Sciences estrogen receptor 2 2-diphenyl-1-picrylhydrazyl DNA damage Black cohosh Cimicifuga racemosa extract Breast Neoplasms chemistry traditional medicine Transfection breast cancer Single-cell gel electrophoresis assay (comet assay) Picrates estrogen receptor alpha comet assay medicine Humans human picric acid scavenger nuclear magnetic resonance spectroscopy methanol cell culture Cimicifuga racemosa isolation and purification Cimicifuga dahurica Plant Extracts human cell plant root Methanol fukinolic acid Biphenyl Compounds Estrogen Receptor alpha DNA General Chemistry Caulophyllum 1 1 diphenyl 2 picrylhydrazyl gene expression phytochemistry genetic transfection Reactive oxygen species Reactive Oxygen Species oxidation reduction reaction ferulic acid DNA Damage |
| Zdroj: | Journal of Agricultural and Food Chemistry J.Agric.Food Chem. |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf020725h |
| Popis: | The roots/rhizomes of Cimicifuga racemosa L. (Nutt.) (black cohosh) have traditionally been used to treat menopausal symptoms through an unknown mechanism of action. In an effort to determine if black cohosh had additional health benefits, methanol extracts were investigated for their potential to scavenge reactive oxygen species and to protect against menadione-induced DNA damage. These extracts effectively scavenged 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals. In addition, the extracts showed dose-dependent decreases in DNA single-strand breaks and oxidized bases induced by the quinone menadione using the comet (single-cell gel electrophoresis assay) and fragment length associated repair enzyme assays, respectively. Bioassay-directed fractionation of the methanolic extracts using the DPPH assay as a monitor led to the isolation of nine antioxidant active compounds: caffeic acid (1), methyl caffeate (2), ferulic acid (3), isoferulic acid (4), fukinolic acid (5), cimicifugic acid A (6), cimicifugic acid B (7), cimicifugic acid F (8), cimiracemate A (9), and cimiracemate B (10). Six of these antioxidants were found to reduce menadione-induced DNA damage in cultured S30 breast cancer cells with the following order of potency: methyl caffeate (2) > caffeic acid (1) > ferulic acid (3) > cimiracemate A (9) > cimiracemate B (10) > fukinolic acid (5). These data suggest that black cohosh can protect against cellular DNA damage caused by reactive oxygen species by acting as antioxidants. 50 7022 7028 Cited By :67 |
| Databáze: | OpenAIRE |
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