Functional characterization of the collagen-binding protein DIP2093 and its influence on host-pathogen interaction and arthritogenic potential of Corynebacterium diphtheriae
| Autor: | Cíntia Silva Santos, Andreas Burkovski, Renata Stavracakis Peixoto, Ana Luíza Mattos-Guaraldi, Elena Hacker, Vanilda Gonçalves Viana, Raphael Hirata, Liliane Simpson Lourêdo, Camila Azevedo Antunes, Jemima F. R. da Silva |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Host–pathogen interaction 030106 microbiology Mutant Microbiology Virulence factor 03 medical and health sciences Mice Bacterial Proteins Cell Line Tumor Animals Humans Adhesins Bacterial Caenorhabditis elegans Corynebacterium diphtheriae biology Binding protein Arthritis Macrophages Diphtheria Epithelial Cells biology.organism_classification Virology Bacterial adhesin RAW 264.7 Cells Host-Pathogen Interactions MSCRAMM Collagen Carrier Proteins Type I collagen HeLa Cells Protein Binding |
| Zdroj: | Microbiology (Reading, England). 163(5) |
| ISSN: | 1465-2080 |
| Popis: | Corynebacterium diphtheriae is typically recognized as the a etiological agent of diphtheria, a toxaemic infection of the respiratory tract; however, both non-toxigenic and toxigenic strains are increasingly isolated from cases of invasive infections. The molecular mechanisms responsible for bacterial colonization and dissemination to host tissues remain only partially understood. In this report, we investigated the role of DIP2093, described as a putative adhesin of the serine-aspartate repeat (Sdr) protein family in host–pathogen interactions of C. diphtheriae wild-type strain NCTC13129. Compared to the parental strain, a DIP2093 mutant RN generated in this study was attenuated in its ability to bind to type I collagen, to adhere to and invade epithelial cells, as well as to survive within macrophages. Furthermore, DIP2093 mutant strain RN had a less detrimental impact on the viability of Caenorhabditis elegans as well as in the clinical severity of arthritis in mice. In conclusion, DIP2093 functions as a microbial surface component recognizing adhesive matrix molecules, and may be included among the factors that contribute to the pathogenicity of C. diphtheriae strains, independently of toxin production. |
| Databáze: | OpenAIRE |
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