Molecular characterisation of osteoblasts from bone obtained from people of Polynesian and European ancestry undergoing joint replacement surgery
| Autor: | Ally J. Choi, Jarome Bentley, Brya G. Matthews, Cluny Macpherson, Ryan Gao, Karen E. Callon, Ian R. Reid, Rocco P. Pitto, Jillian Cornish, Gregory D. Gamble, Anne Horne, Dorit Naot, Usha Bava |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Native Hawaiian or Other Pacific Islander Microarray Science common Population 030209 endocrinology & metabolism Biology White People Article Andrology 03 medical and health sciences Medical research 0302 clinical medicine Polynesians Gene expression medicine Humans Arthroplasty Replacement education Cells Cultured Aged Bone mineral education.field_of_study Osteoblasts Multidisciplinary Molecular medicine Gene Expression Profiling Cell Cycle Computational Biology Cell Differentiation Osteoblast Middle Aged Fold change Gene Ontology 030104 developmental biology medicine.anatomical_structure DKK1 common.group Medicine Female Biomarkers New Zealand |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Population studies in Aotearoa New Zealand found higher bone mineral density and lower rate of hip fracture in people of Polynesian ancestry compared to Europeans. We hypothesised that differences in osteoblast proliferation and differentiation contribute to the differences in bone properties between the two groups. Osteoblasts were cultured from bone samples obtained from 30 people of Polynesian ancestry and 25 Europeans who had joint replacement surgeries for osteoarthritis. The fraction of cells in S-phase was determined by flow cytometry, and gene expression was analysed by microarray and real-time PCR. We found no differences in the fraction of osteoblasts in S-phase between the groups. Global gene expression analysis identified 79 differentially expressed genes (fold change > 2, FDR P COL1A1 and KRT34 in Polynesians, whereas BGLAP, DKK1, NOV, CDH13, EFHD1 and EFNB2 were higher in Europeans (P ≤ 0.01). Osteoblasts from European donors had higher levels of late differentiation markers and genes encoding proteins that inhibit the Wnt signalling pathway. This variability may contribute to the differences in bone properties between people of Polynesian and European ancestry that had been determined in previous studies. |
| Databáze: | OpenAIRE |
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