Selecting the Best: Evolutionary Engineering of Chemical Production in Microbes
Autor: | Rebecca M. Lennen, Hao Luo, Anne Sofie Lærke Hansen, Denis Shepelin, Markus J. Herrgård |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Evolutionary engineering Computer science Mutagenesis (molecular biology technique) Rational engineering Review Growth coupling Chemical production evolutionary engineering Metabolic engineering 03 medical and health sciences Genetics Production (economics) growth coupling Genetics (clinical) Organism genetic engineering Bioproduction bioproduction 030104 developmental biology ALE Genetic engineering Biochemical engineering metabolic engineering |
Zdroj: | Genes Shepelin, D, Hansen, A S L, Lennen, R, Luo, H & Herrgård, M J 2018, ' Selecting the Best: Evolutionary Engineering of Chemical Production in Microbes ', Genes, vol. 9, no. 5, 249 . https://doi.org/10.3390/genes9050249 |
ISSN: | 2073-4425 |
DOI: | 10.3390/genes9050249 |
Popis: | Microbial cell factories have proven to be an economical means of production for many bulk, specialty, and fine chemical products. However, we still lack both a holistic understanding of organism physiology and the ability to predictively tune enzyme activities in vivo, thus slowing down rational engineering of industrially relevant strains. An alternative concept to rational engineering is to use evolution as the driving force to select for desired changes, an approach often described as evolutionary engineering. In evolutionary engineering, in vivo selections for a desired phenotype are combined with either generation of spontaneous mutations or some form of targeted or random mutagenesis. Evolutionary engineering has been used to successfully engineer easily selectable phenotypes, such as utilization of a suboptimal nutrient source or tolerance to inhibitory substrates or products. In this review, we focus primarily on a more challenging problem-the use of evolutionary engineering for improving the production of chemicals in microbes directly. We describe recent developments in evolutionary engineering strategies, in general, and discuss, in detail, case studies where production of a chemical has been successfully achieved through evolutionary engineering by coupling production to cellular growth. |
Databáze: | OpenAIRE |
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