Enzyme activity of horseradish peroxidase in surfactant-free microemulsions

Autor: Werner Kunz, Didier Touraud, Thomas Zinn, Pierre Bauduin, Sebastian Krickl
Přispěvatelé: University Hospital Regensburg, Universität Regensburg (UR), Ions aux Interfaces Actives (L2IA), Institut de Chimie Séparative de Marcoule (ICSM - UMR 5257), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), European Synchrotron Radiation Facility (ESRF), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
tert-Butyl Alcohol
Surface Properties
1-Propanol
02 engineering and technology
010402 general chemistry
01 natural sciences
Horseradish peroxidase
Biomaterials
Propanol
Surface-Active Agents
chemistry.chemical_compound
Colloid and Surface Chemistry
Microemulsion
Benzothiazoles
Particle Size
Hydrogen peroxide
Horseradish Peroxidase
ComputingMilieux_MISCELLANEOUS
Aqueous solution
ABTS
biology
Water
Hydrogen Peroxide
Buffer solution
[CHIM.MATE]Chemical Sciences/Material chemistry
Hydrogen-Ion Concentration
021001 nanoscience & nanotechnology
Enzyme assay
0104 chemical sciences
Surfaces
Coatings and Films
Electronic
Optical and Magnetic Materials
Kinetics
chemistry
biology.protein
Emulsions
Sulfonic Acids
0210 nano-technology
Hydrophobic and Hydrophilic Interactions
Oxidation-Reduction
Limonene
Nuclear chemistry
Zdroj: Journal of Colloid and Interface Science
Journal of Colloid and Interface Science, Elsevier, 2018, 516, pp.466-475. ⟨10.1016/j.jcis.2018.01.077⟩
Journal of Colloid and Interface Science, 2018, 516, pp.466-475. ⟨10.1016/j.jcis.2018.01.077⟩
ISSN: 0021-9797
1095-7103
DOI: 10.1016/j.jcis.2018.01.077⟩
Popis: In the present contribution, we investigated the influence of the structuring of surfactant-free microemulsions (SFME) (water/1-propanol/limonene and water/tert-butanol/limonene) on the enzyme activity of horseradish peroxidase (HRP). To this purpose, the oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) with hydrogen peroxide was chosen as a model reaction. Enzymatic activities in SFMEs of varying compositions were investigated by UV-Vis spectroscopy and compared to the enzyme activity in pure buffer solution. Dynamic light, small-angle-X-ray scattering and conductivity measurements were performed in order to obtain structural information on the used SFMEs. Findings presented in this study revealed that the ability of short-chain alcohols to form mesostructures (aqueous aggregates in oil) has a crucial effect on the enzyme activity in SFMEs. Mesoscale structuring with 1-propanol (NPA) was found to be more pronounced than for the more hydrophobic tert-butanol (TBA). It was concluded that the most pronounced mesoscale-structured SFMEs lead to the highest enzymatic activities.
Databáze: OpenAIRE
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