In vitro transcription from cauliflower mosaic virus promoters by a cell-free extract from tobacco cells
| Autor: | Paul Penon, Richard Cooke |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Transcription
Genetic Molecular Sequence Data RNA polymerase II Plant Science Cell-free system Mosaic Viruses Transcription (biology) Tobacco Gene expression Genetics Tobacco mosaic virus Humans Promoter Regions Genetic Base Sequence Cell-Free System biology Chromosome Mapping Tobamovirus Promoter DNA General Medicine Plants biology.organism_classification Molecular biology Plants Toxic biology.protein RNA RNA Polymerase II Cauliflower mosaic virus Agronomy and Crop Science HeLa Cells |
| Zdroj: | Plant Molecular Biology. 14:391-405 |
| ISSN: | 1573-5028 0167-4412 |
| Popis: | We have studied transcription from the cauliflower mosaic virus 19S and 35S promoters in a cell-free system derived from tobacco cells in suspension culture. While a whole-cell extract is incapable of detectable transcription from these promoters, successive purification by column chromatography allows the preparation of two fractions which contain all factors necessary for transcription from the 19S promoter. In contrast, transcription from the 35S promoter leads to the accumulation of short RNAs. This accumulation can only be partially alleviated by modifying the conditions of transcription. |
| Databáze: | OpenAIRE |
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