The neuroprotective effect of rat adipose tissue-derived mesenchymal stem cell-conditioned medium on cortical neurons using an in vitro model of SCI inflammation
| Autor: | Eva Szekiova, Lucia Slovinska, Juraj Blasko, Jana Plsikova, Dasa Cizkova |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Cell Survival Primary Cell Culture Adipose tissue Inflammation In Vitro Techniques Neuroprotection In vitro model 03 medical and health sciences 0302 clinical medicine medicine Conditioned medium Animals Rats Wistar Spinal Cord Injuries Cerebral Cortex Neurons Chemistry Mesenchymal stem cell Mesenchymal Stem Cells General Medicine Cortical neurons Myelitis Spinal cord Cell biology Disease Models Animal 030104 developmental biology medicine.anatomical_structure Neuroprotective Agents Neurology Adipose Tissue Culture Media Conditioned Neurology (clinical) medicine.symptom Microtubule-Associated Proteins 030217 neurology & neurosurgery |
| Zdroj: | Neurological research. 40(4) |
| ISSN: | 1743-1328 |
| Popis: | Objectives In this study, a new approach was used with an in vitro model in which neural cells were exposed to conditioned media from the injured spinal cord (SCI-CM) mimicking a local inflammatory microenvironment . Subsequently, the neuroprotective effect of rat adipose tissue-derived msesenchymal stem cell-conditioned media (ATMSC-CM) was investigated through a cell-free based therapy, which was used to treat cortical neurons and astrocytes under inflammation. Methods Primary cell cultures isolated from postnatal day (P6) Wistar rat brain cortex were exposed to SCI-CM derived from the central lesion, rostral and caudal segments of injured spinal cord. After 48 h incubation, the SCI-CM was replaced and primary cultures were cultivated either in DMEM media alone or in ATMSC-CM for 72 h. The impact of ATMSC-CM on the viability of neurons and astrocytes was assessed using a CyQUANT® Direct Cell Proliferation Assay Kit as well as immunocytochemistry analysis. Results Immunocytochemical analysis revealed significant decrease in the number of MAP2 positive neurons exposed to SCI-CM compared to Control. Protection by ATMSC-CM was associated with increased survival of neurons compared to primary culture cultivated in DMEM media alone. The ATMSC-CM effect on astrocytes was more variable and without any significant impact. Conclusion The results demonstrate that SCI-CM mimicking inflammation can reduce cortical neuron survival, and subsequent exposure to ATMSC-CM can stabilize the neuronal population most likely via released neuroprotective and trophic factors. In addition, astrogliosis was not affected by ATMSC-CM. |
| Databáze: | OpenAIRE |
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