Thermoplasmonic neural chip platform for in situ manipulation of neuronal connections in vitro
| Autor: | Yoonkey Nam, Nari Hong |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
In situ Neurite Computer science Science Primary Cell Culture Metal Nanoparticles General Physics and Astronomy Topology (electrical circuits) 02 engineering and technology Hippocampus Neural circuits Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Neurites Biological neural network Animals Nanotechnology Cells Cultured Cultured neuronal network Network model Nanotubes Multidisciplinary Artificial neural network Hydrogels Nanobiotechnology General Chemistry Embryo Mammalian 021001 nanoscience & nanotechnology Chip Rats 030104 developmental biology Feasibility Studies Gold Nerve Net 0210 nano-technology Biomedical engineering Neuroscience |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Cultured neuronal networks with a controlled structure have been widely studied as an in vitro model system to investigate the relationship between network structure and function. However, most cell culture techniques lack the ability to control network structures during cell cultivation, making it difficult to assess functional changes induced by specific structural changes. In this study, we present an in situ manipulation platform based on gold-nanorod-mediated thermoplasmonics to interrogate an in vitro network model. We find that it is possible to induce new neurite outgrowths, eliminate interconnecting neurites, and estimate functional relationships in matured neuronal networks. This method is expected to be useful for studying functional dynamics of neural networks under controlled structural changes. Cultured neuron networks provide insight into network structure and function, but the ability to control network topology is a challenge. Here the authors develop a nanorod-mediated thermoplasmonics platform that enables the formation of new connections, the abolishment of existing connections, and the modulation of network activity during cultivation. |
| Databáze: | OpenAIRE |
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