A novel mouse model for segmental orthotopic colon cancer
| Autor: | Ralph Weissleder, Martin Funovics, Charles Stout, Herlen Alencar, Ray King, Umar Mahmood |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Noninvasive imaging Colorectal cancer Green Fluorescent Proteins Mice Nude Colonoscopy Mice Transgenic Lesion Mice Colon carcinoma Genes Reporter medicine Animals Intestinal Mucosa Tumor location Invasive carcinoma medicine.diagnostic_test business.industry Magnetic resonance imaging medicine.disease Magnetic Resonance Imaging Disease Models Animal Oncology Colonic Neoplasms Female medicine.symptom business |
| Zdroj: | International Journal of Cancer. 117:335-339 |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/ijc.21185 |
| Popis: | Spontaneous colon tumor mouse strains offer numerous advantages in modeling disease. However, the wide temporal window in which lesions form and the stochastic nature of lesion location require larger cohorts for assessment of disease modulation. Reliable, reproducible and inexpensive mouse models of early-stage and invasive cancer would add to existing transgenic models. We show a new method for the creation of orthotopic murine tumors centered in the mucosal and submucosal layers anywhere in the colon, allowing creation of lesions of known age, location and extent. The system overcomes the disadvantages of heterotopic implantation and allows evaluation of lesions distally in the colon as well as proximally, thereby providing an additional method to study the effects of regionality. Invasion, host vascularization and application to disparate cell lines are demonstrated. Noninvasive imaging with magnetic resonance and colonoscopy, allowed in part by the tumor location, show potential applications of this approach. |
| Databáze: | OpenAIRE |
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