BMP‐15 activity on in vitro development of collared peccary ( Pecari tajacu Linnaeus, 1758) preantral follicles
| Autor: | Moacir Franco de Oliveira, Lívia Batista Campos, Alexandre Rodrigues Silva, Alexsandra Fernandes Pereira, Erica C.G. Praxedes, Márcia V. A. Saraiva, Henrique A. N. Gomes |
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| Rok vydání: | 2020 |
| Předmět: |
Pecari
Nucleolus Cell Culture Techniques Andrology 03 medical and health sciences Tissue culture 0302 clinical medicine Endocrinology Ovarian Follicle Follicular phase medicine Animals Artiodactyla 030219 obstetrics & reproductive medicine biology 0402 animal and dairy science Collared peccary 04 agricultural and veterinary sciences biology.organism_classification Oocyte 040201 dairy & animal science In vitro medicine.anatomical_structure Female Animal Science and Zoology Folliculogenesis Bone Morphogenetic Protein 15 Biotechnology |
| Zdroj: | Reproduction in Domestic Animals. 55:958-964 |
| ISSN: | 1439-0531 0936-6768 |
| DOI: | 10.1111/rda.13735 |
| Popis: | This study investigated the effects of BMP-15 on the in vitro development of preantral follicles of collared peccaries. Ovarian fragments were cultured for 1 or 6 days in Tissue Culture Medium 199 (TCM199+ ) supplemented with BMP-15 at rates of 0, 1, 25 or 50 ng/ml. The fragments were analysed histologically by evaluating follicular morphology, activation and growth as well as the potential for proliferation of granulosa cells. Our results show the addition of 25 ng/ml BMP-15 in the medium provided the greatest percentage of normal follicles (79.67% ± 0.69) when compared to other treatments (p .05). Moreover, 25 and 50 ng/ml of BMP-15 promoted follicular activation. BMP-15 supplements did not affect oocyte and follicular growth. All concentrations of BMP-15 increased the number of nucleolus organizer regions (NORs) after 1 day of culture when compared to fresh fragments or the control samples (p .05). In summary, the addition of 25 ng/ml BMP-15 to the culture medium of collared peccary preantral follicles maintained a high number of morphologically healthy follicles and stimulated the activation of primordial follicles after 6 days in culture. |
| Databáze: | OpenAIRE |
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