Expression Profile of microRNAs Regulating Proliferation and Differentiation in Mouse Adult Cardiac Stem Cells
| Autor: | Luis Bras-Rosario, Ana Isabel Pinheiro, Alex Matsuda, Rui Gardner, Margarida Gama-Carvalho, Telma Lopes, Andreia J. Amaral |
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| Přispěvatelé: | Repositório da Universidade de Lisboa |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Mouse
Cellular differentiation lcsh:Medicine Gene Expression Stem cells Mice RNA interference Nucleic Acids Molecular Cell Biology Cell differentiation lcsh:Science Muscle differentiation Multidisciplinary Stem Cells Cell Differentiation Heart Animal Models Flow Cytometry Cell biology Endothelial stem cell Adult Stem Cells P19 cell Fluorescence-activated cell sorting Epigenetics Stem cell Cellular Types Adult stem cell Research Article Embryonic stem cells Biology Cell Growth Model Organisms Cancer stem cell Genetics Cell Adhesion Animals Cell Proliferation DNA Primers Gene Expression Profiling Myocardium Mesenchymal stem cell lcsh:R Mesenchymal Stem Cells Embryonic stem cell MicroRNAs Gene Expression Regulation RNA lcsh:Q Gene expression Developmental Biology |
| Zdroj: | PLoS ONE Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP PLoS ONE, Vol 8, Iss 5, p e63041 (2013) |
| Popis: | © 2013 Brás Rosário et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The identification of cardiac cells with stem cell properties changed the paradigm of the heart as a post mitotic organ. These cells proliferate and differentiate into cardiomyocytes, endothelial and vascular smooth muscle cells, providing for cardiac cell homeostasis and regeneration. microRNAs are master switches controlling proliferation and differentiation, in particular regulating stem cell biology and cardiac development. Modulation of microRNAs -regulated gene expression networks holds the potential to control cell fate and proliferation, with predictable biotechnologic and therapeutic applications. To obtain insights into the regulatory networks active in cardiac stem cells, we characterized the expression profile of 95 microRNAs with reported functions in stem cell and tissue differentiation in mouse cardiac stem cells, and compared it to that of mouse embryonic heart and mesenchymal stem cells. The most highly expressed microRNAs identified in cardiac stem cells are known to target key genes involved in the control of cell proliferation and adhesion, vascular function and cardiomyocyte differentiation. We report a subset of differentially expressed microRNAs that are proposed to act as regulators of differentiation and proliferation of adult cardiac stem cells, providing novel insights into active gene expression networks regulating their biological properties. |
| Databáze: | OpenAIRE |
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