Skim milk powder used as a non-permeable cryoprotectant reduces oxidative and DNA damage in cryopreserved zebrafish sperm
| Autor: | Ana Amélia Nunes Fossati, Verônica Bidinotto Brito, Carla I. G. Vogel, Dinara Jaqueline Moura, Ana Carina Nogueira Vasconcelos, Danilo Pedro Streit, Antonio Cesar Godoy, Rômulo Batista Rodrigues, Mariana Uczay |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
food.ingredient Cryoprotectant DNA damage Balanced salt solution medicine.disease_cause General Biochemistry Genetics and Molecular Biology Cryopreservation 03 medical and health sciences Cryoprotective Agents 0302 clinical medicine food Skimmed milk medicine Animals Dimethyl Sulfoxide Food science Zebrafish 030219 obstetrics & reproductive medicine Chemistry 0402 animal and dairy science 04 agricultural and veterinary sciences General Medicine Spermatozoa 040201 dairy & animal science Sperm Comet assay Oxidative Stress Milk Sperm Motility Powders General Agricultural and Biological Sciences Oxidative stress DNA Damage Semen Preservation |
| Zdroj: | Cryobiology. 97:76-84 |
| ISSN: | 0011-2240 |
| DOI: | 10.1016/j.cryobiol.2020.10.005 |
| Popis: | Cryoprotectants play a vital role in the cryopreservation process, protecting biological samples from freezing damage. Here, we evaluate the effects of the combination and interaction of different extenders with permeable and non-permeable cryoprotectants, on the cryopreservation of Danio rerio sperm, analyzing the effects of cryopreservation through a broad approach to variables. Two extenders were used, Hank's balanced salt solution (HBSS) and Ginsburg's solution. Eight cryoprotective solutions (CS) were used: CS1 (HBSS + Me2SO 8%), CS2 (HBSS + Methanol 8%), CS3 (HBSS + Me2SO 8% + Skim milk powder 15%), CS4 (HBSS + Methanol 8% + Skim milk powder 15%), CS5 (Ginsburg + Me2SO 8%), CS6 (Ginsburg + Methanol 8%), CS7 (Ginsburg + Me2SO 8% + Skim milk powder 15%) and CS8 (Ginsburg + Methanol 8% + Skim milk powder 15%). The samples were cryopreserved in cryovials for 20 min on dry ice, stored in liquid nitrogen, thawed at 38 °C for 10 s, and analyzed. In addition to increasing viability, we show that powdered milk also allows for better preservation of the membrane and normal cell morphology, and protects the sperm cells from DNA damage and oxidative stress caused by cryopreservation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect