MicroRNA Signatures of Colonic Polyps on Screening and Histology
| Autor: | Vassiliki L. Tsikitis, Stanley R. Hamilton, Amiee Potter, Christina A. Harrington, Angela N. Bartley, Achyut K. Bhattacharyya, M. Peter Lance, Patricia A. Thompson, Julie A. Buckmeier, Christina Preece, Motomi Mori |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Adenoma
Male Risk 0301 basic medicine Cancer Research Pathology medicine.medical_specialty Colorectal cancer Colonic Polyps Colonoscopy Biology Article 03 medical and health sciences 0302 clinical medicine Biopsy medicine Humans Early Detection of Cancer Aged medicine.diagnostic_test Gene Expression Profiling Cancer Middle Aged medicine.disease digestive system diseases MicroRNAs Logistic Models 030104 developmental biology Oncology Hyperplastic Polyp Dysplasia 030220 oncology & carcinogenesis Female Neoplasm Grading Colorectal Neoplasms Sessile serrated adenoma |
| Zdroj: | Cancer Prevention Research. 9:942-949 |
| ISSN: | 1940-6215 1940-6207 |
| DOI: | 10.1158/1940-6207.capr-16-0086 |
| Popis: | Colorectal cancer and adenoma adjacent to cancer exhibit distinct microRNA (miRNA) alterations in an apparent mucosa-to-adenocarcinoma sequence. The pattern of microRNAs in screen-detected polyps in relation to histologic features and cancer risk has not been investigated. miRNA expression analysis was performed on normal mucosa (NM), hyperplastic polyps (HP), tubular adenomas (TA), tubulovillous adenomas or high-grade dysplasia (TVHG), and serrated polyps [sessile serrated adenoma/polyps (SSA/P) and traditional serrated adenomas (TSA)] in biopsy specimens from 109 patients undergoing screening/surveillance colonoscopy. Generalized linear models were used to identify differentially expressed miRNAs by histologic type and logistic regression to identify miRNA predictors of histopathology. False discovery rate (FDR) was used to control for multiple comparisons. We identified 99 miRNAs differing in at least one of five histopathologic groups (FDR ≤0.05). In a comparison of HPNM versus TVHG, the top most upregulated and downregulated miRNAs in HPNM included miR-145, -143, -107, -194, and -26a (upregulated), and miR-663, -1268, -320b, -1275, and -320b (downregulated; FDR P < 0.05). miR-145 and -619 showed high accuracy to discriminate low- from high-risk polyps without serrated histology (TVHG vs. HPNM + TA; CI, 95.6%), whereas miR-124, -143, and -30a showed high accuracy of separating high-risk polyps (TVHG + TSA) from low-risk polyps (HPNM + TA + SSA/P; CI, 96.0%). For TSAs, miR-125b and -199a were uniquely downregulated relative to HPNMs, and miR-335, -222, and -214 discriminated between non-serrated and serrated histology. Our data support the presence of colorectal cancer–associated miRNA alterations in screen-detected adenomas that may be useful for risk stratification for surveillance interval planning. Cancer Prev Res; 9(12); 942–9. ©2016 AACR. |
| Databáze: | OpenAIRE |
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