Injectable gellan gum-based nanoparticles-loaded system for the local delivery of vancomycin in osteomyelitis treatment
| Autor: | Elżbieta Pamuła, Monika Brzychczy-Włoch, Urszula Posadowska |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Materials science
Cell Survival Staphylococcus Dispersity Biomedical Engineering Biophysics Nanoparticle Bioengineering Microbial Sensitivity Tests 02 engineering and technology 010402 general chemistry 01 natural sciences Cell Line Biomaterials chemistry.chemical_compound Capillary electrophoresis Polylactic Acid-Polyglycolic Acid Copolymer Dynamic light scattering Vancomycin Humans Lactic Acid Osteoblasts Polysaccharides Bacterial Special Issue: ESB 2015 Osteomyelitis 021001 nanoscience & nanotechnology Antimicrobial Gellan gum Anti-Bacterial Agents 0104 chemical sciences Lactic acid chemistry Chemical Engineering(all) Nanoparticles Extrusion 0210 nano-technology Polyglycolic Acid Biomedical engineering |
| Zdroj: | Journal of Materials Science. Materials in Medicine |
| Popis: | Infection spreading in the skeletal system leading to osteomyelitis can be prevented by the prolonged administration of antibiotics in high doses. However systemic antibiotherapy, besides its inconvenience and often low efficacy, provokes numerous side effects. Thus, we formulated a new injectable nanoparticle-loaded system for the local delivery of vancomycin (Vanc) applied in a minimally-invasive way. Vanc was encapsulated in poly(Llactide- co-glycolide) nanoparticles (NPs) by double-emulsification. The size (258 ± 11 nm), polydispersity index (0.240 ± 0.003) and surface potential (-25.9 ± 0.2 mV) of NPs were determined by dynamic light scattering and capillary electrophoresis measurements. They have a spherical morphology and a smooth topography as observed using atomic force microscopy. Vanc loading and encapsulation efficiencies were 8.8 ± 0.1 and 55.2 ± 0.5 %, respectively, based on fluorescence spectroscopy assays. In order to ensure injectability, NPs were suspended in gellan gum and cross-linked with $Ca^{2+}$; also a portion of dissolved antibiotic was added to the system. The resulting system was found to be injectable (extrusion force 11.3 ± 1.1 N), reassembled its structure after breaking as shown by rheology tests and ensured required burst release followed by sustained Vanc delivery. The system was cytocompatible with osteoblast-like MG-63 cells (no significant impact on cells’ viability was detected). Growth of Staphylococcus spp. reference strains and also those isolated from osteomyelitic joints was inhibited in contact with the injectable system. As a result we obtained a biocompatible system displaying ease of application (low extrusion force), self-healing ability after disruption, adjustable drug release and antimicrobial properties. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|