Corona protein composition and cytotoxicity evaluation of ultra-small zeolites synthesized from template free precursor suspensions
| Autor: | Morteza Mahmoudi, Eng-Poh Ng, Coralie Thirifays, Sophie Laurent, Carmen Burtea, G. M. Goupil, M. M. Motazacker, de Marcel Vries, Cécile Hébert, Louwanda Lakiss, Emad Oveisi, Farhad Rezaee, Svetlana Mintova |
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| Přispěvatelé: | NMR and Molecular Imaging Laboratory [Mons], University of Mons [Belgium] (UMONS), Laboratoire de Matériaux à Porosité Contrôlée (LMPC), Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Ecole Nationale Supérieure de Chimie de Mulhouse-Centre National de la Recherche Scientifique (CNRS), Laboratoire catalyse et spectrochimie (LCS), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Université de Caen Normandie (UNICAEN), Normandie Université (NU), Ecole Polytechnique Fédérale de Lausanne (EPFL), Nexio, Roland, Pascal, ACS - Amsterdam Cardiovascular Sciences, Human Genetics |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
MECHANISM
Health Toxicology and Mutagenesis NANOZEOLITES Nanoparticle 02 engineering and technology 010402 general chemistry Toxicology Molecular sieve 01 natural sciences TOXICITY [CHIM] Chemical Sciences NANOPARTICLES Organic chemistry [CHIM]Chemical Sciences Viability assay Zeolite Cytotoxicity chemistry.chemical_classification Chemistry Biomolecule IN-VITRO 021001 nanoscience & nanotechnology 0104 chemical sciences SIZE Nanocrystal Chemical engineering Nanotoxicology 0210 nano-technology NANOTOXICOLOGY |
| Zdroj: | Toxicology Research Toxicology Research, Royal Society of Chemistry, 2013, 2 (4), pp.270--279 TOXICOLOGY RESEARCH, 2(4), 270-279. Royal Society of Chemistry Toxicology research, 2(4), 270-279. ROYAL SOC CHEMISTRY |
| ISSN: | 2045-452X 2045-4538 |
| DOI: | 10.1039/c3tx50023c |
| Popis: | International audience; The toxicity of two types of ultra-small zeolites (8-18 nm) with LTL-and EMT-type structures is reported. Both the LTL- and EMT-type zeolites belong to the same group of molecular sieves; they have large pores (7.1-7.5 angstrom) and low silica content (Si/Al = 1.2-2.3). The zeolites are prepared by an environmentally friendly synthetic approach from precursor suspensions without using any organic template. Cellular interactions with the two types of zeolite nanocrystals are evaluated by cell viability, reactive oxygen species and cell life cycle assays. It is found that various concentrations of zeolites have negligible effects on the cell life cycle. Moreover, the LTL- and EMT-types zeolites did not cause extensive oxidative stress on the cells. Although it is seen that the zeolites extensively entered in the cells, there is no sign of toxicity for all employed concentrations of ultra-small EMT and LTL zeolites. Additionally, no abnormality in DNA replication while exposed to the zeolites is observed. Very importantly, the zeolite corona shows a high affinity for fibrinogen, moderate affinity for apoA-II and complement factor 3, and trace affinity for albumin, which is the most abundant protein of human plasma. Thus the zeolite nanoparticles can be considered as very promising material for purification of fibrinogen and lipoproteins. Since fibrinogen is considered as acute phase protein and found to be the most associated biomolecule in the composition of corona at the surface of zeolites, we propose that these nanoparticles can be potentially pro-inflammatory for in vivo applications. |
| Databáze: | OpenAIRE |
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