Neuronal Regulation of Myelin Basic Protein mRNA Translocation in Oligodendrocytes Is Mediated by Platelet-Derived Growth Factor
| Autor: | Vilma Schonmann, Anthony T. Campagnoni, Shashi Amur-Umarjee |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Platelet-derived growth factor
medicine.medical_treatment Blotting Western Cell Count Biology medicine.disease_cause Translocation Genetic Receptor tyrosine kinase Mice chemistry.chemical_compound Developmental Neuroscience Protein targeting medicine Animals RNA Messenger Remyelination Fluorescent Antibody Technique Indirect Cells Cultured In Situ Hybridization Neurons Platelet-Derived Growth Factor Mice Inbred BALB C Messenger RNA Growth factor Myelin Basic Protein Protein-Tyrosine Kinases Molecular biology Myelin basic protein Oligodendroglia medicine.anatomical_structure Neurology chemistry Astrocytes biology.protein Platelet-derived growth factor receptor Signal Transduction |
| Zdroj: | Developmental Neuroscience. 19:143-151 |
| ISSN: | 1421-9859 0378-5866 |
| DOI: | 10.1159/000111200 |
| Popis: | Translocation of mRNAs has emerged as an important form of protein targeting. In oligodendrocytes, the myelin-forming cells of the central nervous system, myelin basic protein (MBP) mRNAs are transported into cell processes and the cytoplasmic channels that infiltrate the myelin sheath. This mRNA movement is important for myelination and occurs in purified oligodendrocytes in vitro, but not in oligodendrocytes grown on bed layers of astrocytes. We have shown previously that this astrocytic inhibition depends on cell-cell contact and is partially relieved in primary cultures that contain some neurons in addition to oligodendrocytes and astrocytes. We report here that soluble factors, secreted by neurons, are responsible for relieving the astrocytic inhibition of MBP mRNA translocation. Of several growth factors tested, only platelet-derived growth factor (PDGF) was effective in alleviating the astrocytic inhibition. Double immunofluorescence analysis demonstrated the presence of PDGF alpha-receptors in oligodendrocytes. PDGF appears to mediate its effect via its alpha-receptors and receptor tyrosine kinases. This interaction among the three neural cell types may play an important role in regulating remyelination after injury. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|