Complete genomic characterization of an intertypic Sabin 3/Sabin 2 capsid recombinant
| Autor: | Zissis Mamuris, Christos Brakoulias, Panayotis Markoulatos, Vaia Pliaka, Stamatina Levidiotou-Stefanou, Evaggelos Dedepsidis, Anastassia Pratti, Z. Kyriakopoulou |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Microbiology (medical)
Immunology Genome Viral Biology medicine.disease_cause complex mixtures Microbiology Genome Virus law.invention Feces Capsid Proteins/analysis/chemistry/*genetics law medicine Immunology and Allergy Coding region Humans Recombinant Proteins/genetics Child Antigens Viral Genetics Recombination Genetic Mutation Antigens Viral/analysis/genetics Poliovirus General Medicine Virology Recombinant Proteins Infectious Diseases Capsid Poliovirus Vaccine Oral Poliovirus/chemistry/*genetics Recombinant DNA Enterovirus Capsid Proteins Poliovirus Vaccine Oral/*genetics/immunology/metabolism Feces/virology |
| Popis: | The genetic properties of strain K/2002, isolated from fecal samples of a 7-month-old child who had received his first oral poliovirus vaccine (OPV) dose at the age of 3 months, are described. Preliminary sequencing characterization of isolate K/2002 revealed an S3/S2 recombination event at the 3' end of the VP1 coding region. A recombination event resulted in the introduction of six Sabin 2 amino acid residues in a Sabin 3 genomic background. Furthermore, mutations associated with loss of the attenuated phenotype of Sabin 3 strains have been identified in the genome of isolate K/2002. The data presented here emphasize the need for careful planning of vaccination strategies, which involve stopping OPV administration in regions that are certified to be polio-free. FEMS Immunol Med Microbiol |
| Databáze: | OpenAIRE |
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