A Combination of MEF3 and NFI Proteins Activates Transcription in a Subset of Fast-Twitch Muscles
Autor: | Josiane Demignon, Axel Kahn, Dominique Daegelen, François Spitz, Pascal Maire, Marjo Salminen |
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Rok vydání: | 1997 |
Předmět: |
Transcriptional Activation
Gene isoform Mef2 Recombinant Fusion Proteins Mice Transgenic Chick Embryo MyoD Gene Expression Regulation Enzymologic Mice Transcription (biology) Fructose-Bisphosphate Aldolase Animals Humans Binding site Promoter Regions Genetic Molecular Biology Transcription factor Reporter gene Binding Sites biology MEF2 Transcription Factors Aldolase A Nuclear Proteins Exons Cell Biology musculoskeletal system Molecular biology Rats DNA-Binding Proteins NFI Transcription Factors Myogenic Regulatory Factors Organ Specificity Muscle Fibers Fast-Twitch CCAAT-Enhancer-Binding Proteins biology.protein Y-Box-Binding Protein 1 Research Article Transcription Factors |
Zdroj: | University of Helsinki |
ISSN: | 1098-5549 |
Popis: | The human aldolase A pM promoter is active in fast-twitch muscles. To understand the role of the different transcription factors which bind to this promoter and determine which ones are responsible for its restricted pattern of expression, we analyzed several transgenic lines harboring different combinations of pM regulatory elements. We show that muscle-specific expression can be achieved without any binding sites for the myogenic factors MyoD and MEF2 and that a 64-bp fragment comprising a MEF3 motif and an NFI binding site is sufficient to drive reporter gene expression in some but, interestingly, not all fast-twitch muscles. A result related to this pattern of expression is that some isoforms of NFI proteins accumulate differentially in fast- and slow-twitch muscles and in distinct fast-twitch muscles. We propose that these isoforms of NFI proteins might provide a molecular basis for skeletal muscle diversity. |
Databáze: | OpenAIRE |
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