A new attempt to distinguish serologically the subspecies of Treponema pallidum causing syphilis and yaws
| Autor: | E. Stolz, R. H. Meloen, Alan Cockayne, J. D. A. Van Embden, Leo M. Schouls, G. T. Noordhoek |
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| Rok vydání: | 1990 |
| Předmět: |
Microbiology (medical)
Serotype medicine.drug_class Molecular Sequence Data Biology urologic and male genital diseases Monoclonal antibody Microbiology Bacterial Proteins Species Specificity Antigen Western blot medicine Humans Amino Acid Sequence Syphilis Treponema pallidum Serotyping Peptide sequence Antigens Bacterial Treponema medicine.diagnostic_test Antibodies Monoclonal bacterial infections and mycoses medicine.disease biology.organism_classification Virology female genital diseases and pregnancy complications Yaws Treponematosis Research Article |
| Zdroj: | Journal of Clinical Microbiology. 28:1600-1607 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | In an effort to serologically differentiate syphilis from yaws, 69 monoclonal antibody species raised against Treponema pallidum subsp. pallidum were tested by immunoblotting for their reactivity with Treponema pallidum subsp. pertenue. All monoclonal antibodies reacted with antigens with the same molecular weight of both subspecies. Furthermore, no differences in reactivity between sera from yaws patients and from syphilis patients were found by Western blot (immunoblot) analysis of cell lysates of T. pallidum subsp. pallidum and T. pallidum subsp. pertenue. We tried to exploit the only known molecular difference between the subspecies. The subunits of the 190-kilodalton multimeric proteins TpF1 and TyF1 of T. pallidum subsp. pallidum and T. pallidum subsp. pertenue, respectively, have previously been shown to differ in one amino acid residue at position 40. In this study, no difference was found in immunoreactivity of TpF1 or TyF1 with either syphilis sera or yaws sera. Synthetic peptides based on the sequence of TpF1 and of TyF1 were used in an enzyme-linked immunosorbent assay with syphilis sera and yaws sera. Again, no difference in reactivity between the T. pallidum subsp. pallidum- and T. pallidum subsp. pertenue-derived peptides was observed. |
| Databáze: | OpenAIRE |
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