Circovirus in Blood of a Febrile Horse with Hepatitis
| Autor: | Caitlin Fletcher, Alvin Hui, Nathan M. Slovis, Eric Delwart, Xutao Deng, Eda Altan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Circovirus
0301 basic medicine Viral metagenomics 040301 veterinary sciences viruses animal diseases Genome Viral Equues caballus Microbiology Article 0403 veterinary science 03 medical and health sciences symbols.namesake Virology Animals hepatitis Horses Viremia Circoviridae Infections Phylogeny Circoviridae Whole genome sequencing Sanger sequencing Genus Equus biology Parvovirus virus diseases Genomics 04 agricultural and veterinary sciences biology.organism_classification QR1-502 Flavivirus 030104 developmental biology Infectious Diseases Hepatitis Viral Animal symbols Horse Diseases viral metagenomics |
| Zdroj: | Viruses Volume 13 Issue 5 Viruses, Vol 13, Iss 944, p 944 (2021) |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v13050944 |
| Popis: | Circoviruses infect vertebrates where they can result in a wide range of disease signs or in asymptomatic infections. Using viral metagenomics we analyzed a pool of five sera from four healthy and one sick horse. Sequences from parvovirus-H, equus anellovirus, and distantly related to mammalian circoviruses were recognized. PCR identified the circovirus reads as originating from a pregnant mare with fever and hepatitis. That horse’s serum was also positive by real time PCR for equine parvovirus H and negative for the flavivirus equine hepacivirus. The complete circular genome of equine circovirus 1 strain Charaf (EqCV1-Charaf) was completed using PCR and Sanger sequencing. EqCV1 replicase showed 73–74% identity to those of their closest relatives, pig circoviruses 1/2, and elk circovirus. The closest capsid proteins were from the same ungulate circoviruses with 62–63% identity. The overall nucleotide identity of 72% to its closest relative indicates that EqCV1 is a new species in the Circovirus genus, the first reported in genus Equus. Whether EqCV1 alone or in co-infections can result in disease and its prevalence in different equine populations will require further studies now facilitated using EqCV1′s genome sequence. |
| Databáze: | OpenAIRE |
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