Industrial light at the end of the iron‐containing (group III) alcohol dehydrogenase tunnel

Autor: Anirudh P. Shanbhag, Arindam Ghatak, Sreenath Rajagopal
Rok vydání: 2022
Předmět:
Zdroj: Biotechnology and Applied Biochemistry. 70:537-552
ISSN: 1470-8744
0885-4513
Popis: There are three prominent alcohol dehydrogenases superfamilies: short-chain, medium-chain, and iron-containing alcohol dehydrogenases (FeADHs). Many members are valuable catalysts for producing industrially relevant products such as active pharmaceutical intermediates, chiral synthons, biopolymers, biofuels, and secondary metabolites. However, FeADHs are the least explored enzymes among the superfamilies for commercial tenacities. They portray a conserved structure having a "tunnel-like" cofactor and substrate binding site with particular functions, despite representing high sequence diversity. Interestingly, phylogenetic analysis demarcates enzymes catalyzing distinct native substrates where closely related clades convert similar molecules. Further, homologs from various mesophilic and thermophilic microbes have been explored for designing a solvent and temperature-resistant enzyme for industrial purposes. The review explores different iron-containing alcohol dehydrogenases potential engineering of the enzymes and substrates helpful in manufacturing commercial products.
Databáze: OpenAIRE
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