The effects of interferon-α and acyclovir on herpes simplex virus type-1 ribonucleotide reductase
| Autor: | Jerry L. Taylor, William J. O'Brien, Jana Narasimhan, Peggy Tom, Julie Guy |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Free Radicals
Protein subunit Blotting Western Acyclovir Alpha interferon Herpesvirus 1 Human Interferon alpha-2 Biology medicine.disease_cause Antiviral Agents Cornea Interferon Virology Chlorocebus aethiops Ribonucleotide Reductases medicine Animals Humans RNA Messenger Vero Cells Cells Cultured Pharmacology chemistry.chemical_classification Electron Spin Resonance Spectroscopy Interferon-alpha Blotting Northern Recombinant Proteins Enzyme assay Enzyme Herpes simplex virus Ribonucleotide reductase chemistry Biochemistry Mechanism of action biology.protein RNA Viral Tyrosine Stromal Cells medicine.symptom medicine.drug |
| Zdroj: | Antiviral Research. 38:107-116 |
| ISSN: | 0166-3542 |
| DOI: | 10.1016/s0166-3542(98)00016-3 |
| Popis: | Herpes simplex virus-type 1 (HSV-1) encodes both the small (UL40) and large (UL39) subunits of the enzyme, ribonucleotide reductase. Treatment of HSV-1-infected cells with interferon-alpha (IFN-alpha) reduced the levels of both enzyme subunits. Reduced steady state levels of the large subunit were demonstrated by immunoblot using polyclonal antibody specific for the viral enzyme. Reduction in the amount of small subunit was shown by a reduction in the electron spin resonance signal derived from the iron-containing tyrosyl free-radical present in this subunit. Treatment of cells with 100 IU/ml of IFN-alpha decreased levels of both subunits resulting in a reduction in enzyme activity as measured by conversion of CDP to dCDP. The decrease in the amount of the large subunit was not due to a reduction in the level of its mRNA. The combination of IFN-alpha and ACV treatment of human cornea stromal cells did not result in a further reduction in amounts of ribonucleotide reductase relative to that detected with IFN-alpha alone. The IFN-alpha-induced reduction in ribonucleotide reductase activity is the likely cause of decreased levels of dGTP which we have previously demonstrated in IFN-alpha-treated, infected cells. |
| Databáze: | OpenAIRE |
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