Tuberculosis transmission by Mycobacterium bovis in a mixed cattle and goat herd
| Autor: | Alessandra Gaffuri, Giorgio Zanardi, Maria Lodovica Pacciarini, Maria Beatrice Boniotti, Barbara Casto, Mariagrazia Zanoni |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
Veterinary medicine Tuberculosis Tuberculin Cattle Diseases Enzyme-Linked Immunosorbent Assay Serology Disease Outbreaks Interferon-gamma medicine Animals Mycobacterium bovis Goat Diseases General Veterinary biology Tuberculin Test Goats Outbreak medicine.disease biology.organism_classification Antibodies Bacterial Variable number tandem repeat biology.protein Herd Cattle Female Antibody |
| Zdroj: | Research in veterinary science. 95(2) |
| ISSN: | 1532-2661 |
| Popis: | A tuberculosis (TB) outbreak caused by Mycobacterium bovis occurred in a mixed herd of three cattle and eighteen goats in Northern Italy in 2005. All the cattle were removed, as opposed to the co-existing goats, who remained in the farm and were not subsequently tested by the official intradermal tuberculin test. At the beginning of May 2006, a 7-day old calf was introduced into the herd from an officially TB-free (OTF) farm. On October 2006, tuberculous lesions were detected at the slaughterhouse in the same animal. The following epidemiological investigation on the herd highlighted a clinical suspicion of TB in one goat out of 35, and visible lesions were found at necropsy in the respiratory and intestinal tracts. Bacteriological culture and molecular tests confirmed the presence of M. bovis in both animals. Spoligotyping and Mycobacterial Interspersed Repetitive Units – Variable Number of Tandem Repeats (MIRU-VNTR) showed the same genomic profile of the previous breakdown occurred in 2005. Since this profile has never been described in Italy, these findings suggest the probable transmission of TB within the farm among cattle and goats. The remaining 34 goats were also tested by single intradermal cervical comparative tuberculin (SICCT) test, Interferon (IFN)-γ assay and ELISA for antibody to M. bovis. The SICCT test and the IFN-γ showed a good concordance with 20 and 19 positive reactors, respectively. By ELISA we found 12 Ab-positive animals, seven of which had not been detected by the tests for cell-mediated immunity. Finally, 15 goats were found positive for gross lesions at necropsy. The in vivo tests revealed a total of 27 positive animals out of 35, which highlights the usefulness of the serology in parallel with SICCT and IFN-γ when an advanced stage of infection is suspected. Moreover, our results confirm the necessity for adopting the official tuberculin test on goats co-existing with cattle. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect