Purification and partial sequence analysis of human interleukin-2 derived from peripheral blood leukocytes
| Autor: | Kyozo Tsukamoto, Kenji Kawahara, Shuji Hinuma, Hiroko Tada, Masaru Koyama, Koichi Kato, K Naruo, Hiromu Sugino |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Interleukin 2
Sequence analysis Biophysics Biology Biochemistry High-performance liquid chromatography Jurkat cells Chromatography DEAE-Cellulose Column chromatography Complementary DNA Leukocytes medicine Humans Amino Acid Sequence Amino Acids Molecular Biology Chromatography High Pressure Liquid chemistry.chemical_classification Cell Biology Molecular biology Amino acid Molecular Weight Isoelectric point chemistry Interleukin-2 Electrophoresis Polyacrylamide Gel medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 127:182-190 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(85)80142-x |
| Popis: | Summary Human peripheral blood leukocyte-derived interleukin-2 (IL-2) was resolved by DEAE-cellulose column chromatography into three peaks of activity, IL-2A, B, and C, with isoelectric points of 7.2, 6.6, and 7.9, respectively. IL-2 A, B, and C were further purified by reverse phase high performance liquid chromatography and resolved into two apparently homogeneous peaks each with identical molecular weight: A-1 and A-2 (Mr17000); B-1 and B-2 (Mr17500); and C-1 and C-2 (Mr14400). The amino acid compositions and partial NH 2 -terminal amino acid sequences of these molecular species were consistent with those predicted from IL-2 cDNA sequences derived from Jurkat and peripheral blood leukocytes. |
| Databáze: | OpenAIRE |
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