Investigation into the effect of microsampling on mouse fetuses and pregnant mice in the embryofetal development study design
| Autor: | Jane Stewart, Sarah Kirk, Terri Mitchard, Claire Grant |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Animal Use Alternatives
0301 basic medicine medicine.medical_specialty Hot Temperature Embryonic Development Physiology Biology Handling Psychological Toxicology Fetal Development Mice 03 medical and health sciences Pregnancy Internal medicine Toxicity Tests medicine Animals Maternal health Adverse effect Blood Specimen Collection Fetus Fetal weight 030104 developmental biology Endocrinology Research Design Gestation Female medicine.symptom Maternal body Weight gain |
| Zdroj: | Reproductive Toxicology. 67:140-145 |
| ISSN: | 0890-6238 |
| DOI: | 10.1016/j.reprotox.2016.12.006 |
| Popis: | The effects on fetal weights and maternal health of taking 32 μL blood microsamples at the end of organogenesis in a mouse embryofetal development (EFD) study design was investigated with the aim of reducing satellite animal usage. The effects of warming, handling and sampling either 3 or 6 times on gestation day 16 was evaluated. Maternal body weight gain was transiently reduced when animals underwent warming and handling with or without microsampling. Fetal weights on gestation day 18 were reduced after 6 occasion warming and handling alone or taking samples, but not when sampling was limited to 3 timepoints. Taking 3 microsamples of 32 μL had no permanent adverse effects on maternal health or toxicologically significant effects on fetal development (measured by fetal weights). This regimen could be used to generate composite toxicokinetic profiles using only 6 main test animals in mouse EFD studies provided sampling procedures were matched across groups. |
| Databáze: | OpenAIRE |
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