Rapid and improved identification of drinking water bacteria using the Drinking Water Library, a dedicated MALDI-TOF MS database
| Autor: | Rosa Aznar, M. Carmen Macián, Francisco Lucena, Cristina García-Aljaro, Susana González, Carles Vilaró, Laia Caudet-Segarra, David Baquero, Anicet R. Blanch, Anna Pinar-Méndez, Lidia Rodrigo-Torres, Sonia Fernández, Belén Galofré, David R. Arahal, María A. Ruvira, Laura Sala-Comorera |
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| Rok vydání: | 2021 |
| Předmět: |
Environmental Engineering
Databases Factual Microbiologia computer.software_genre Bacteris Water Purification Identification rate Human health Escherichia coli Drinking water Humans Waste Management and Disposal Water Science and Technology Civil and Structural Engineering Database biology Bacteria Ecological Modeling Drinking Water Water Bacteria Present Bottled water biology.organism_classification Pollution Aigua potable Matrix-assisted laser desorption/ionization Aigua Escheríchia coli Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Environmental science Water treatment Water quality computer |
| Zdroj: | Dipòsit Digital de la UB Universidad de Barcelona Pinar-Méndez, Anna Fernández, Sonia Baquero, David Vilaró, Carles Galofré, Belén González, Susana Rodrigo-Torres, Lidia Arahal, David R. Macián Rovira, M. Carmen Ruvira, María A. Aznar Novella, Rosa Caudet-Segarra, Laia Sala-Comorera, Laura Lucena, Francisco Blanch, Anicet R. Garcia-Aljaro, Cristina 2021 Rapid and improved identification of drinking water bacteria using the Drinking Water Library, a dedicated MALDI-TOF MS database Water Research 203 117543 RODERIC. Repositorio Institucional de la Universitat de Valéncia instname |
| ISSN: | 1879-2448 |
| Popis: | According to the European Directives (UE) 2020/2184 and 2009/54/EC, which establishes the sanitary criteria for water intended for human consumption in Europe, water suitable for human consumption must be free of the bacterial indicators Escherichia coli, Clostridium perfringens and Enterococcus spp. Drinking water is also monitored for heterotrophic bacteria, which are not a human health risk, but can serve as an index of bacteriological water quality. Therefore, a rapid, accurate, and cost-effective method for the identification of these colonies would improve our understanding of the culturable bacteria of drinking water and facilitate the task of water management by treatment facilities. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is potentially such a method, although most of the currently available mass spectral libraries have been developed in a clinical setting and have limited environmental applicability. In this work, a MALDI-TOF MS drinking water library (DWL) was defined and developed by targeting bacteria present in water intended for human consumption. This database, made up of 319 different bacterial strains, can contribute to the routine microbiological control of either treated drinking water or mineral bottled water carried out by water treatment and distribution operators, offering a faster identification rate compared to a clinical sample-based library. The DWL, made up of 96 bacterial genera, 44 of which are not represented in the MALDI-TOF MS bacterial Bruker Daltonics (BDAL) database, was found to significantly improve the identification of bacteria present in drinking water. |
| Databáze: | OpenAIRE |
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