OsGL1-3 is involved in cuticular wax biosynthesis and tolerance to water deficit in rice
| Autor: | Faxi Xu, Jianhua Xiang, Xiangyuan Wan, Yan Peng, Linzhi Li, Xinbo Chen, Ailing Liu, Xianwen Zhang, Xiaoyun Zhou, Guofu Gao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Chlorophyll
Cell Membrane Permeability Gene Expression lcsh:Medicine Plant Science Genetically modified crops Plant Genetics Biochemistry Plant Epidermis chemistry.chemical_compound Plant Growth Regulators Gene Expression Regulation Plant Plant Resistance to Abiotic Stress lcsh:Science Abscisic acid Phylogeny Genetic Interference Plant Proteins Wax Multidisciplinary Ecology biology Reverse Transcriptase Polymerase Chain Reaction Plant Biochemistry food and beverages Plants Genetically Modified Adaptation Physiological Droughts Plant Physiology visual_art visual_art.visual_art_medium RNA Interference Research Article Biotechnology Drought Adaptation Drought tolerance Oryza Molecular Genetics Plant-Environment Interactions Botany Genetics Molecular Biology Crop Genetics Plant Ecology fungi lcsh:R Membrane Proteins Water Biology and Life Sciences Plant Transpiration biology.organism_classification Genetically modified rice Plant Leaves chemistry Seedlings Seedling Waxes Microscopy Electron Scanning Plant Biotechnology lcsh:Q Gene Function Abscisic Acid |
| Zdroj: | PLoS ONE, Vol 10, Iss 1, p e116676 (2015) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Cuticular wax covers aerial organs of plants and functions as the outermost barrier against non-stomatal water loss. We reported here the functional characterization of the Glossy1(GL1)-homologous gene OsGL1-3 in rice using overexpression and RNAi transgenic rice plants. OsGL1-3 gene was ubiquitously expressed at different level in rice plants except root and its expression was up-regulated under ABA and PEG treatments. The transient expression of OsGL1-3–GFP fusion protein indicated that OsGL1-3 is mainly localized in the plasma membrane. Compared to the wild type, overexpression rice plants exhibited stunted growth, more wax crystallization on leaf surface, and significantly increased total cuticular wax load due to the prominent changes of C30–C32 aldehydes and C30 primary alcohols. While the RNAi knockdown mutant of OsGL1-3 exhibited no significant difference in plant height, but less wax crystallization and decreased total cuticular wax accumulation on leaf surface. All these evidences, together with the effects of OsGL1-3 on the expression of some wax synthesis related genes, suggest that OsGL1-3 is involved in cuticular wax biosynthesis. Overexpression of OsGL1-3 decreased chlorophyll leaching and water loss rate whereas increased tolerance to water deficit at both seedling and late-tillering stages, suggesting an important role of OsGL1-3 in drought tolerance. |
| Databáze: | OpenAIRE |
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