Detection of trace amounts of abamectin used as an antiparasitic agent in fallow deer tissues
| Autor: | Gabrijela Tavčar-Kalcher, Silvestra Kobal, Andrej Bidovec, Diana Žele, Gorazd Vengušt, Anton Vengušt |
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| Rok vydání: | 2009 |
| Předmět: |
Detection limit
Chromatography Ivermectin General Veterinary Pesticide residue Trace Amounts Antiparasitic Agents Deer Trifluoroacetic acid anhydride Kidney High-performance liquid chromatography Antiparasitic agent chemistry.chemical_compound chemistry Adipose Tissue Abamectin Animals Tissue distribution Muscle Skeletal Chromatography High Pressure Liquid |
| Zdroj: | Acta veterinaria Hungarica. 57(1) |
| ISSN: | 0236-6290 |
| Popis: | A sensitive and reliable method for the determination of trace amounts of abamectin in muscles, kidneys and fat tissue of fallow deer is presented. Abamectin was extracted from the tissues with acetonitrile and the extract was cleaned up on a C8 solid-phase extraction cartridge. Abamectin residue was derivatised with trifluoroacetic acid anhydride and 1-methylimidazole, and determined using reversed- phase high-performance liquid chromatography under isocratic conditions and fluorescence detection. The recoveries of the method were high and consistent, ranging from 78% to 90%. The limit of detection of the method was below 1 μg/kg when analysing muscle, kidney and fat tissue. Matrix-matched calibration was used in order to obtain accurate values and to avoid matrix interference. |
| Databáze: | OpenAIRE |
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