ATR-FTIR spectroscopy and spectroscopic imaging for the analysis of biopharmaceuticals
| Autor: | Sergei G. Kazarian, Bernadette Byrne, Hannah Tiernan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Diagnostic Imaging
Future studies Atr ftir spectroscopy Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences Article Protein Structure Secondary Biopharmaceuticals Analytical Chemistry Protein content Aggregation Spectroscopy Fourier Transform Infrared Research based Fourier transform infrared spectroscopy Spectroscopy Biosimilar Pharmaceuticals Instrumentation 0306 Physical Chemistry (incl. Structural) Chemical Physics Bioprocessing Chemistry Proteins 0303 Macromolecular and Materials Chemistry 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics 0104 chemical sciences FTIR spectroscopy ATR Attenuated total reflection 0210 nano-technology 0301 Analytical Chemistry |
| Zdroj: | Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy |
| ISSN: | 1386-1425 |
| DOI: | 10.1016/j.saa.2020.118636 |
| Popis: | Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy is a label-free, non-destructive technique that can be applied to a vast range of biological applications, from imaging cancer tissues and live cells, to determining protein content and protein secondary structure composition. This review summarises the recent advances in applications of ATR-FTIR spectroscopy to biopharmaceuticals, the application of this technique to biosimilars, and the current uses of FTIR spectroscopy in biopharmaceutical production. We discuss the use of ATR-FTIR spectroscopic imaging to investigate biopharmaceuticals, and finally, give an outlook on the possible future developments and applications of ATR-FTIR spectroscopy and spectroscopic imaging to this field. Throughout the review comparisons will be made between FTIR spectroscopy and alternative analytical techniques, and areas will be identified where FTIR spectroscopy could perhaps offer a better alternative in future studies. This review focuses on the most recent advances in the field of using ATR-FTIR spectroscopy and spectroscopic imaging to characterise and evaluate biopharmaceuticals, both in industrial and academic research based environments. Graphical abstract Unlabelled Image Highlights • This review article summarises the recent advances in applications of ATR-FTIR spectroscopy to biopharmaceuticals, the application of this technique to biosimilars, and the current uses of FTIR spectroscopy in biopharmaceutical production. |
| Databáze: | OpenAIRE |
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