Double-stranded RNA adenosine deaminase ADAR-1-induced hypermutated genomes among inactivated seasonal influenza and live attenuated measles virus vaccines

Autor: Vincent Petit, Rodolphe Suspène, Jean-Pierre Vartanian, Denise Guetard, Simon Wain-Hobson, Michel Henry, David Puyraimond-Zemmour, Christophe Rusniok, Marie-Ming Aynaud
Přispěvatelé: Rétrovirologie Moléculaire, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Biologie des bactéries intracellulaires, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Adenosine Deaminase
MESH: Measles Vaccine
MESH: Trypsin
MESH: Protein Structure
Secondary
MESH: Amino Acid Sequence
MESH: Base Sequence
medicine.disease_cause
MESH: Influenza Vaccines
Chlorocebus aethiops
Influenza A virus
MESH: Animals
MESH: Peptide Fragments
MESH: Adenosine Deaminase
0303 health sciences
biology
MESH: Influenza
Human
030302 biochemistry & molecular biology
RNA-Binding Proteins
3. Good health
Virus-Cell Interactions
MESH: Rabies virus
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Influenza Vaccines
MESH: Measles
Seasons
MESH: Molecular Chaperones
MESH: Genome
Viral
MESH: Mutation
Immunology
Measles Vaccine
Molecular Sequence Data
MESH: Influenza A virus
MESH: Sequence Alignment
MESH: Vero Cells
Context (language use)
Genome
Viral
MESH: Viral Structural Proteins
Vaccines
Attenuated
MESH: Two-Hybrid System Techniques
Microbiology
Measles
MESH: Phosphoproteins
Virus
Cell Line
Measles virus
03 medical and health sciences
Virology
Influenza
Human
MESH: Vaccines
Attenuated
medicine
Animals
Humans
MESH: Protein Binding
Vero Cells
030304 developmental biology
MESH: Humans
MESH: Molecular Sequence Data
Base Sequence
MESH: Immunoprecipitation
MESH: Protein Interaction Mapping
MESH: Nucleocapsid Proteins
medicine.disease
biology.organism_classification
MESH: Cercopithecus aethiops
MESH: Cell Line
Cell culture
Insect Science
ADAR
Mutation
Measles vaccine
MESH: Measles virus
MESH: Seasons
Zdroj: Journal of Virology
Journal of Virology, American Society for Microbiology, 2011, 85 (5), pp.2458-62. ⟨10.1128/JVI.02138-10⟩
Journal of Virology, 2011, 85 (5), pp.2458-62. ⟨10.1128/JVI.02138-10⟩
ISSN: 0022-538X
1098-5514
DOI: 10.1128/JVI.02138-10⟩
Popis: We sought to examine ADAR-1 editing of measles and influenza virus genomes derived from inactivated seasonal influenza and live attenuated measles virus vaccines grown on chicken cells as the culture substrate. Using highly sensitive 3DI-PCR (R. Suspène et al., Nucleic Acids Res. 36:e72, 2008), it was possible to show that ADAR-1 could hyperdeaminate adenosine residues in both measles virus and influenza virus A genomes. Detailed analysis of the dinucleotide editing context showed preferences for 5′ArA and 5′UrA, which is typical of editing in mammalian cells. The hyperedited mutant frequency, including genomes and antigenomes, was a log greater for influenza virus compared to measles virus, suggesting a greater sensitivity to restriction by ADAR-1.
Databáze: OpenAIRE
Externí odkaz: