Chronic inhalation of diesel exhaust and coal dust: effect of age and exposure on selected enzyme activities associated with microsomal cytochrome P-450 in rat lung and liver
Autor: | Jean Rabovsky, Karl J. Marion, Trent R. Lewis, Robert D. Groseclose, Martin R. Petersen |
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Rok vydání: | 1984 |
Předmět: |
Male
medicine.medical_specialty Diesel exhaust Toxicology Coal dust complex mixtures Benzopyrene Hydroxylase Cytochrome P-450 Enzyme System Internal medicine Microsomes medicine Animals Lung Vehicle Emissions Analysis of Variance Inhalation biology Chemistry Age Factors Exhaust gas Cytochrome P450 Dust respiratory system Pollution Rats Inbred F344 respiratory tract diseases Rats Endocrinology Coal Toxicity Microsome biology.protein Microsomes Liver |
Zdroj: | Journal of toxicology and environmental health. 14(5-6) |
ISSN: | 0098-4108 |
Popis: | Male Fisher-344 rats were exposed by inhalation to low levels of diesel exhaust and coal dust, alone or in combination, or to filtered air, 7 h/d, 5 d/wk for 24 mo. Cytochrome P-450-associated benzo[a]pyrene hydroxylase and 7-ethoxycoumarin deethylase activities were assayed in lung and liver microsomes after 3, 6, and 24 mo. Age-related changes in enzyme activities were observed, but they were not altered by the exposures. When the data were adjusted for age, only one difference was observed. Lung benzo[a]pyrene hydroxylase activity in rats exposed to diesel exhaust and coal dust in combination was lower than that in animals exposed to coal dust alone (2.8 versus 4.4 pmol/min X mg protein). Neither value, however, differed significantly from the filtered-air controls, and no differences were observed in the other lung and liver activities. The data suggest exposure of the rats to diesel exhaust and/or coal dust had little or no effect on the selected lung and liver cytochrome P-450 activities under the conditions of the experiment. |
Databáze: | OpenAIRE |
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