Inhibitory effect and mechanism of exogenous mammalian sterile 20-like kinase 1 on the growth of human colorectal cancer
| Autor: | Liqiao Liu, Hongfei Lu, Baohua Xu, Fusheng Wan, Jian Wu, Bo Yu, Xiaohong Yang, Shuangyan Zheng, Kemin Jie |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research colorectal cancer Biology medicine.disease_cause human mammalian sterile 20-like kinase 1 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine p53 upregulated modulator of apoptosis Viability assay Oncogene Kinase apoptosis Articles Cell cycle gene therapy 030104 developmental biology Oncology chemistry yes-associated protein Apoptosis 030220 oncology & carcinogenesis biology.protein Cancer research Growth inhibition Carcinogenesis |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1082 1792-1074 |
| DOI: | 10.3892/ol.2017.5786 |
| Popis: | The present study aimed to observe the inhibitory effect and preliminary mechanism of exogenous mammalian sterile 20-like kinase 1 (MST1) on the growth of colorectal cancer SW480 cells. The SW480 cells were randomly divided into the following groups: Control, empty enhanced green fluorescent protein (EGFP) plasmid (pEGFP-N1), MST1 EGFP plasmid (pEGFP-MST1), 20 µmol/l fluorouracil (5-FU) and pEGFP-MST1 + 5-FU. An MTS colorimetric assay was used to detect cell viability, Hoechst 33342 staining was used to observe cell apoptosis, and western blotting and immunohistochemistry were used to detect the levels of the proteins MST1, yes-associated protein (YAP), phospho-YAP1 (Ser127), p53 and p53 upregulated modulator of apoptosis (PUMA). In addition, nude mice were injected with SW480 cells to assess the tumor inhibition rates. Compared with the control group, the growth inhibition and apoptosis rates, the levels of MST1, p53 and PUMA, and the ratios of phospho-YAP1/YAP in the pEGFP-MST1 and pEGFP-MST1 + 5-FU groups were increased significantly (P |
| Databáze: | OpenAIRE |
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