Modulation of CTP: Phosphocholine cytidylyltransferase translocation by oleic acid and the antitumoral alkylphospholipid in HL-60 cells
| Autor: | Gérard Ribbes, Hugues Chap, Michel Record, Hélène Tronchère, François Tercé |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Choline kinase
Cytidylyltransferase Biophysics Phospholipid Antineoplastic Agents Oleic Acids Biology Biochemistry Cell Line Choline Choline-phosphate cytidylyltransferase chemistry.chemical_compound Cytosol Leukemia Promyelocytic Acute Phosphatidylcholine Humans Choline-Phosphate Cytidylyltransferase Molecular Biology Phosphocholine Cell-Free System Cell Membrane Phospholipid Ethers Cell Biology Nucleotidyltransferases Cytidylyltransferase activity Kinetics chemistry Phosphatidylcholines Protein Processing Post-Translational Oleic Acid |
| Zdroj: | Biochemical and Biophysical Research Communications. 176:157-165 |
| ISSN: | 0006-291X |
| Popis: | Short time effect of oleate and 1-O-alkyl-2-O-methyl-rac-glycero-3-phosphocholine (AMGPC) on choline incorporation into phosphatidylcholines were studied in HL-60 cells. The non lytic concentration of 50 microM oleate induced a three-fold increase in [3H]choline incorporation into phosphatidylcholine. This stimulation was accompanied by a translocation of the CTP:phosphocholine cytidylyltransferase (EC 2.7.7.15) from cytosol to membranes. By contrast, the ether-lipid AMGPC inhibited [3H]choline incorporation into phosphatidylcholine by 60% at 10 microM. AMGPC had no effect on choline kinase or choline phosphotransferase activities. When AMGPC was added separately to an homogenate, a particulate or a cytosolic fraction, cytidylyltransferase inhibition was observed only in the homogenate. However on particulates recovered from homogenates treated with increasing concentrations of AMGPC, membranous cytidylyltransferase activity decreased dose-dependently. Thus AMGPC had no effect on cytidylyltransferase activity itself but inhibited its translocation from cytosol to membrane. At variance with the well-established positive effect on cytidylyltransferase translocation induced by fatty acids, this is the first demonstration that AMGPC can inhibit cytidylyltransferase translocation in cell-free system. |
| Databáze: | OpenAIRE |
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