A Model of a Synthetic Biological Communication Interface between Mammalian Cells and Mechatronic Systems
| Autor: | Warren C. Ruder, Keith C. Heyde |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering Biology Bioinformatics ENCODE Models Biological Signal Mice 03 medical and health sciences Fluorescence microscope Animals Humans Gene Regulatory Networks Calcium Signaling Electrical and Electronic Engineering Calcium signaling Robotics Mechatronics Computer Science Applications 030104 developmental biology Frequency domain Robot Synthetic Biology Signal transduction Biological system Biotechnology |
| Zdroj: | IEEE Transactions on NanoBioscience. 15:864-870 |
| ISSN: | 1558-2639 1536-1241 |
| DOI: | 10.1109/tnb.2016.2620942 |
| Popis: | The creation of communication interfaces between abiotic and biotic systems represents a significant research challenge. In this work, we design and model a system linking the biochemical signaling pathways of mammalian cells to the actions of a mobile robotic prosthesis. We envision this system as a robotic platform carrying an optically monitored bioreactor that harbors mammalian cells. The cellular, optical signal is captured by an onboard fluorescent microscope and converted into an electronic signal. We first present a design for the overall cell-robot system, with a specific focus on the design of the synthetic gene networks needed for the system. We use these synthetic networks to encode motion commands within the cell's endogenous, oscillatory calcium signaling pathways. We then describe a potential system whereby this oscillatory signal could be outputted and monitored as a change in cellular fluorescence. Next, we use the changes resulting from the synthetic biological modifications as new parameters in a simulation of a well-established mathematical model for intracellular calcium signaling. The resulting signal is processed in the frequency domain, with specific frequencies activating cognate robot motion subroutines. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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