A soluble factor induced by an extract from Pinus parviflora Sieb et Zucc can inhibit the replication of human immunodeficiency virus in vitro
Autor: | Akiko Tanaka, Y Tamura, K Konno, W G Bradley, P K Lai, M Nonoyama |
---|---|
Rok vydání: | 1991 |
Předmět: |
Antineoplastic Agents
Virus Replication Polymerase Chain Reaction Cell Line Trees Humans Macrophage Pinus parviflora Growth Substances Interleukin 3 Multidisciplinary biology Plant Extracts Tumor Necrosis Factor-alpha Lymphokine Transforming growth factor beta biology.organism_classification Virology Molecular biology Recombinant Proteins In vitro Cell culture HIV-1 biology.protein Interleukin-3 Tumor necrosis factor alpha Research Article |
Zdroj: | Proceedings of the National Academy of Sciences. 88:2249-2253 |
ISSN: | 1091-6490 0027-8424 |
Popis: | We showed that an extract (PC6) from cones of Pinus parviflora Sieb et Zucc induced the human T-cell line CEM to produce a pepsin-sensitive soluble factor(s) that could inhibit the replication of the type 1 human immunodeficiency virus (HIV-1) in CEM T cells, in U-937 histocytes, in THP-1 monocytes, and in mitogen-activated human tonsillar mononuclear cells. Indirect immunofluorescence staining and polymerase chain reaction analysis of the PC6-induced CEM cells revealed the absence of known lymphokines/cytokines except granulocyte/macrophage colony-stimulating factor (GM-CSF), interleukin 3 (IL-3), transforming growth factor beta 1 (TGF-beta 1), and tumor necrosis factor alpha (TNF-alpha). However, functional studies with recombinant IL-3, TNF-alpha, and TGF-beta 1 showed that these three factors did not inhibit HIV-1 replication in CEM cells. Neutralization of the PC6-induced HIV-1-inhibiting factor(s) with commercially available neutralizing antibodies to GM-CSF and TNF-alpha also did not abrogate the anti-HIV-1 impact. Thus, the anti-HIV-1 factor induced by PC6 may be novel. Molecular sieve separation showed that the anti-HIV-1 factor(s) is smaller than 30 kDa. Affinity chromatography using a DEAE-cellulose column enriched the factor that inhibited HIV-1. |
Databáze: | OpenAIRE |
Externí odkaz: |