omega-Conotoxin-sensitive Ca2+ voltage-gated channels modulate protein secretion in cultured rat Sertoli cells
| Autor: | Anna Taranta, Anna Rita Morena, Elena Barbacci, Angela D'Agostino |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
endocrine system Sertoli Cells Voltage-gated ion channel Proteins Biology Sertoli cell Biochemistry Cell biology Rats Endocrinology Secretory protein medicine.anatomical_structure FGF9 omega-Conotoxin GVIA medicine Animals Conotoxin Calcium Channels Rats Wistar Peptides Molecular Biology Calcium influx Ion Channel Gating Total protein |
| Zdroj: | Molecular and cellular endocrinology. 126(2) |
| ISSN: | 0303-7207 |
| Popis: | Recent results have demonstrated that substantial calcium influx in rat Sertoli cells is mediated by cation channels of both L- and N-type. In this report we have investigated the possible role of such channels in the protein secretion of immature rat Sertoli cell monolayers. The blocking of N-type voltage-gated channels by ω-conotoxin (ω-CTX) GVIA results in a 50–60% inhibition of the protein secretion in the culture medium while total protein and RNA synthesis are not affected. The same extent of protein secretion inhibition is obtained in FSH-stimulated Sertoli cells. L-type voltage-gated channels apparently are not involved in such a modulation. These data, showing that a major fraction of secreted proteins from cultured rat Sertoli cells is Ca2+ dependent, represent the first evidence of a physiological role of voltage-operated Ca2+ channels in mammalian testis. |
| Databáze: | OpenAIRE |
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