| Popis: |
Supplementary Fig. S3Analysis of the desynchronization of colonies of cells expressing FastFUCCI. a, FUCCI simulation for a single, synchronized colony with 4 cells. At a given time point, all cells in the colony will all either be green or not. b, simulating multiple colonies gives rise to multimodal distributions of green cell percentage in colonies. c, adding a phase shift parameter allows cells in each colony to be out of sync, producing percentages of green cells in each colony other than 0% or 100% for a given observation point. d, by modifying the simulation parameters, various frequency distributions of the percentage of green cells can be obtained for several colonies. Note that a, b and c represent three specific time steps of a full simulation run, which must walk all cells around the cell cycle at least once, and produced a final distribution considering all evaluated time steps. |
