Serum-free culturing of human mesenchymal stem cells with immobilized growth factors
| Autor: | Hongli Mao, Seong Min Kim, Masashi Ueki, Yoshihiro Ito |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
food.ingredient medicine.medical_treatment media_common.quotation_subject Basic fibroblast growth factor Biomedical Engineering 02 engineering and technology Biology Gelatin 03 medical and health sciences chemistry.chemical_compound food medicine General Materials Science Internalization media_common Cell growth Growth factor Mesenchymal stem cell General Chemistry General Medicine 021001 nanoscience & nanotechnology Cell biology Chemically defined medium 030104 developmental biology chemistry Cell culture Immunology 0210 nano-technology |
| Zdroj: | Journal of Materials Chemistry B. 5:928-934 |
| ISSN: | 2050-7518 2050-750X |
| DOI: | 10.1039/c6tb02867e |
| Popis: | In vitro expansion of human mesenchymal stem cells (hMSCs) using serum-free culture medium is important for basic research and clinical applications. It is known that some growth factors are required for developing a defined medium for hMSC culture. However, growth factors usually show poor stability, short circulating half-life and a rapid rate of cellular internalization when they are in a diffusible state. A potential way to overcome these problems is to immobilize growth factors on materials. Here, three different types of growth factors, basic fibroblast growth factor, transforming growth factor-beta, and platelet-derived growth factor, were co-immobilized on cell culture dish surfaces by photo-reactive gelatin and used for serum-free hMSC cultures. The results showed that the immobilized growth factors supported cell proliferation similarly to the serum-containing medium. More importantly, the immobilization of growth factors significantly improved their thermal stability and efficiently prolonged their shelf life at 4 °C and 37 °C. Furthermore, the immobilized growth factors could be reused at least three times without losing their stimulation effect on cell proliferation. This photo-reactive gelatin-based immobilization of growth factors appears to be a promising method for serum-free hMSC culturing. |
| Databáze: | OpenAIRE |
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