Olfactory marker protein (OMP) regulates formation and refinement of the olfactory glomerular map

Autor: Prateek Agarwal, Allison C. Provost, Venkatesh N. Murthy, Dinu F. Albeanu, Edward R. Soucy, Joseph D. Zak
Rok vydání: 2023
Předmět:
0301 basic medicine
Science
General Physics and Astronomy
Biology
Receptors
Odorant
urologic and male genital diseases
complex mixtures
Olfactory Receptor Neurons
Article
General Biochemistry
Genetics and Molecular Biology
Genetic Heterogeneity
Mice
03 medical and health sciences
0302 clinical medicine
Olfactory Marker Protein
medicine
Animals
lcsh:Science
Receptor
Alleles
Uncategorized
Mice
Knockout
Glomerulus (olfaction)
Multidisciplinary
urogenital system
Genetic heterogeneity
General Chemistry
respiratory system
Immunohistochemistry
Olfactory Bulb
Mice
Mutant Strains
Sensory neuron
Olfactory bulb
Cell biology
030104 developmental biology
medicine.anatomical_structure
Odor
nervous system
biology.protein
bacteria
lcsh:Q
sense organs
Signal transduction
Olfactory marker protein
030217 neurology & neurosurgery
Zdroj: Nature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
Nature Communications
DOI: 10.25417/uic.19346753
Popis: Inputs from olfactory sensory neuron (OSN) axons expressing the same type of odorant receptor (OR) converge in the glomerulus of the main olfactory bulb. A key marker of mature OSNs is olfactory marker protein (OMP), whose deletion has been associated with deficits in OSN signal transduction and odor discrimination. Here, we investigate glomerular odor responses and anatomical architecture in mice in which one or both alleles of OMP are replaced by the fluorescent synaptic activity reporter, synaptopHluorin. Functionally heterogeneous glomeruli, that is, ones with microdomains with distinct odor responses, are rare in OMP+/– mice, but occur frequently in OMP–/– mice. Genetic targeting of single ORs reveals that these microdomains arise from co-innervation of individual glomeruli by OSNs expressing different ORs. This glomerular mistargeting is locally restricted to a few glomerular diameters. Our studies document functional heterogeneity in sensory input within individual glomeruli and uncover its anatomical correlate, revealing an unexpected role for OMP in the formation and refinement of the glomerular map.
Olfactory marker protein (OMP) expressed in all olfactory sensory neurons (OSN) is required for proper signal transduction and odor discrimination. Here, the authors report that OMP deletion leads to formation of glomeruli with axons from heterogeneous OSNs due to local axonal mistargeting.
Databáze: OpenAIRE
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