The Initial Cardiac Tissue Response to Cryopreserved Allogeneic Adipose Tissue-Derived Mesenchymal Stromal Cells in Rats with Chronic Ischemic Cardiomyopathy
| Autor: | Simon Bentsen, Morten Juhl, Lisbeth D. Højgaard, Cecilie Hoeeg, Andreas Kjaer, Ingrid Hunter, Annette Ekblond, Kristina B. V. Døssing, Jens Kastrup, Rasmus S. Ripa, Kaya B. Lund, Carsten H. Nielsen, Bjarke Follin |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Pathology Angiogenesis Macrophage Myocardial Infarction Myocardial Ischemia Adipose tissue heart failure Cell therapy Medicine Biology (General) Spectroscopy Cells Cultured mesenchymal stem cell Mesenchymal stem cell General Medicine Computer Science Applications Chemistry medicine.anatomical_structure Mode of action medicine.medical_specialty Stromal cell QH301-705.5 Heart failure macrophage Mesenchymal Stem Cell Transplantation Catalysis Article Inorganic Chemistry MSC mode of action Animals Physical and Theoretical Chemistry Molecular Biology QD1-999 Cardioimmunology Cryopreservation Ischemic cardiomyopathy business.industry Monocyte Allogeneic Cells Organic Chemistry Mesenchymal Stem Cells Rats Rats Inbred Lew cell therapy cardioimmunology business |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 21 Follin, B, Hoeeg, C, Højgaard, L D, Juhl, M, Lund, K B, Døssing, K B V, Bentsen, S, Hunter, I, Nielsen, C H, Ripa, R S, Kastrup, J, Ekblond, A & Kjaer, A 2021, ' The initial cardiac tissue response to cryopreserved allogeneic adipose tissue-derived mesenchymal stromal cells in rats with chronic ischemic cardiomyopathy ', International Journal of Molecular Sciences, vol. 22, no. 21, 11758 . https://doi.org/10.3390/ijms222111758 International Journal of Molecular Sciences, Vol 22, Iss 11758, p 11758 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms222111758 |
| Popis: | Mesenchymal stromal cells have proven capable of improving cardiac pump function in patients with chronic heart failure, yet little is known about their mode of action. The aim of the study was to investigate the short-term effect of cryopreserved allogeneic rat adipose tissue-derived stromal cells (ASC) on cardiac composition, cellular subpopulations, and gene transcription in a rat model of chronic ischemic cardiomyopathy (ICM). Myocardial infarction (MI) was induced by permanent ligation of the left anterior descending coronary artery. After 6 weeks, the rats were treated with ASCs, saline, or no injection, using echo-guided trans-thoracic intramyocardial injections. The cardiac tissue was subsequently collected for analysis of cellular subpopulations and gene transcription 3 and 7 days after treatment. At day 3, an upregulation of genes associated with angiogenesis were present in the ASC group. On day 7, increases in CCR2+ and CD38+ macrophages (p = 0.047 and p = 0.021), as well as in the CD4/CD8 lymphocyte ratio (p = 0.021), were found in the ASC group compared to the saline group. This was supported by an upregulation of genes associated with monocytes/macrophages. In conclusion, ASC treatment initiated an immune response involving monocytes/macrophages and T-cells and induced a gene expression pattern associated with angiogenesis and monocyte/macrophage differentiation. |
| Databáze: | OpenAIRE |
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