Sensitive and Specific PCR Systems for Detection of Both Chinese and Japanese Severe Fever with Thrombocytopenia Syndrome Virus Strains and Prediction of Patient Survival Based on Viral Load
| Autor: | Hideki Tani, Koji Yano, Hideo Osako, Masayuki Shimojima, Hirokazu Takimoto, Y. Shimazu, Hiroaki Kitamoto, Yasuhiro Nishino, Shoichi Toda, Shuetsu Fukushi, Akira Yoshikawa, Katsuyuki Ando, Shigeru Morikawa, Tomoki Yoshikawa, Tsuyoshi Kuzuguchi, Fumie Suzuki, Ken Maeda, Takumi Motoya, Takuya Yamagishi, Kouji Kida, Toru Takahashi, Miki Kan, Nobuyuki Kato, Aiko Fukuma, Toshiharu Morimitsu, Masayuki Saijo, Kazunori Oishi, Takahiro Yumisashi, Satoshi Taniguchi |
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| Rok vydání: | 2014 |
| Předmět: |
Phlebovirus
Microbiology (medical) Bunyaviridae Infections Real-Time Polymerase Chain Reaction Virus Japan Virology Case fatality rate medicine Humans Retrospective Studies biology Reverse Transcriptase Polymerase Chain Reaction SFTS virus Viral Load Prognosis biology.organism_classification medicine.disease Severe fever with thrombocytopenia syndrome Blood Real-time polymerase chain reaction Molecular Diagnostic Techniques RNA Viral Viral load Severe fever with thrombocytopenia syndrome virus |
| Zdroj: | Journal of Clinical Microbiology. 52:3325-3333 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease with a high case fatality risk and is caused by the SFTS virus (SFTSV). A retrospective study conducted after the first identification of an SFTS patient in Japan revealed that SFTS is endemic to the region, and the virus exists indigenously in Japan. Since the nucleotide sequence of Japanese SFTSV strains contains considerable differences compared with that of Chinese strains, there is an urgent need to establish a sensitive and specific method capable of detecting the Chinese and Japanese strains of SFTSV. A conventional one-step reverse transcription-PCR (RT-PCR) (cvPCR) method and a quantitative one-step RT-PCR (qPCR) method were developed to detect the SFTSV genome. Both cvPCR and qPCR detected a Chinese SFTSV strain. Forty-one of 108 Japanese patients suspected of having SFTS showed a positive reaction by cvPCR. The results from the samples of 108 Japanese patients determined by the qPCR method were in almost complete agreement with those determined by cvPCR. The analyses of the viral copy number level in the patient blood samples at the acute phase determined by qPCR in association with the patient outcome confirmed that the SFTSV RNA load in the blood of the nonsurviving patients was significantly higher than that of the surviving patients. Therefore, the cvPCR and qPCR methods developed in this study can provide a powerful means for diagnosing SFTS. In addition, the detection of the SFTSV genome level by qPCR in the blood of the patients at the acute phase may serve as an indicator to predict the outcome of SFTS. |
| Databáze: | OpenAIRE |
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