Determination of the Antioxidant Activity of Samples of Tea and Commercial Sources of Vitamin C, Using an Enzymatic Biosensor
| Autor: | Djanira Rubim Dos Santos, Andressa Rose Castro Costa, Mihaela Badea, Gabriela Santos Silva, Eliane Braga Ribeiro, Gilvanda Silva Nunes, Danilo Braga Ribeiro |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Antioxidant Physiology medicine.medical_treatment Clinical Biochemistry vitamin C 01 natural sciences Biochemistry Article drugs 03 medical and health sciences chemistry.chemical_compound medicine Molecular Biology Detection limit chemistry.chemical_classification Prussian blue Chromatography Vitamin C teas Biomolecule lcsh:RM1-950 010401 analytical chemistry Cell Biology Ascorbic acid biosensors 0104 chemical sciences lcsh:Therapeutics. Pharmacology 030104 developmental biology Membrane antioxidants chemistry Biosensor xanthine oxidase |
| Zdroj: | Antioxidants Volume 10 Issue 2 Antioxidants, Vol 10, Iss 324, p 324 (2021) |
| ISSN: | 2076-3921 |
| Popis: | Antioxidants are synthetic or natural compounds capable of preventing or delaying oxidative damage caused by chemical species that can oxidize cell biomolecules, such as proteins, membranes, and DNA, leading to the development of various pathologies, such as cancer, atherosclerosis, Parkinson, Alzheimer, and other diseases serious. In this study, an amperometric biosensor was used to determine the antioxidant activity of teas and effervescent products based on vitamin C, available on the market. A sensor composed of three electrodes was used. The performance of the following electrochemical mediators was evaluated: meldola blue combined with Reineck salt (MBRS), Prussian blue (PB), and cobalt phthalocyanine (CoPC), as well as the time of polymerization in the enzymatic immobilization process and the agitation process during chronoamperometric measurements. Prussian blue proved to be more efficient as a mediator for the desired purposes. After optimizing the construction stages of the biosensor, as well as the operational parameters, it presented stability for a period of 7 months. The results clearly indicate that the biosensor can be successfully used to detect fraud in products called “antioxidants” or even in drugs containing less ascorbic acid than indicated on the labels. The detection limit was set at 4.93 µmol·L−1. |
| Databáze: | OpenAIRE |
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